Exercise-induced extracellular 72 kDa heat shock protein (Hsp72) stimulates neutrophil phagocytic and fungicidal capacities via TLR-2
Exercise-induced extracellular 72 kDa heat shock protein (Hsp72) stimulates neutrophil phagocytic and fungicidal capacities via TLR-2
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DOI：
10.1007/s00421-009-1201-8
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Ortega, Eduardo
中科院分区：
文献类型：
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作者：
Giraldo, Esther;Martin-Cordero, Leticia;Ortega, Eduardo
本研究评估了toll样受体2 （TLR-2）在72 kDa细胞外热休克蛋白（Hsp72，一种应激诱导蛋白）与中性粒细胞相互作用中的作用，以及TLR-2在运动后生理浓度的Hsp72刺激中性粒细胞吞噬和杀真菌能力中的作用。用异硫氰酸荧光素偶联的Hsp72培养人外周血中性粒细胞，用免疫荧光显微镜和流式细胞术分析。两种方法均显示Hsp72与中性粒细胞相互作用。此外，当中性粒细胞与抗tlr -2抗体预孵育时，这种相互作用明显减少。运动后循环Hsp72浓度（8.6 ng/ml）刺激中性粒细胞的吞噬和杀真菌能力，这种作用也可以用TLR-2抗体阻断。磷脂酰肌醇-3激酶（PI3K）、细胞外信号调节激酶（ERK）和核转录因子kappa β (NF-kappa I-2)参与了这一信号传导过程，证实了TLR-2参与了Hsp72对中性粒细胞功能的刺激。总之，TLR-2至少在一定程度上参与了运动后生理浓度Hsp72诱导的中性粒细胞吞噬和杀真菌能力的刺激。
This study evaluated the role of toll like receptor 2 (TLR-2) in the interaction of 72 kDa extracellular heat shock protein (Hsp72, a stress-inducible protein) with neutrophils and the participation on TLR-2 in the stimulation of neutrophil phagocytic and fungicidal capacities by post-exercise physiological concentrations of Hsp72. Human peripheral blood neutrophils were incubated with fluorescein isothiocyanate-conjugated Hsp72, and were analyzed by immunofluorescence microscopy and flow cytometry. Both methods revealed an interaction of Hsp72 with neutrophils. In addition, when neutrophils were pre-incubated with an anti-TLR-2 antibody this interaction was clearly decreased. Post-exercise circulating concentration of Hsp72 (8.6 ng/ml) stimulated the phagocytic and fungicidal capacities of neutrophils and this effect could be also blocked using an antibody against TLR-2. Phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated kinase (ERK) and the nuclear transcription factor kappa beta (NF-kappa I-2) were found to be involved in the signaling process, confirming the participation of TLR-2 in the stimulation of neutrophil function by Hsp72. In conclusion, TLR-2 is involved at least in part, in the stimulation of neutrophil phagocytic and fungicidal capacities induced by post-exercise physiological concentrations of Hsp72.