Increase in bFGF-responsive neural progenitor population following contusion injury of the adult rodent spinal cord
Increase in bFGF-responsive neural progenitor population following contusion injury of the adult rodent spinal cord
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DOI：
10.1016/j.neulet.2005.12.051
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发表时间：
2006-04
影响因子：
2.5
通讯作者：
Yi Xu;M. Kitada;M. Yamaguchi;M. Dezawa;C. Idé
中科院分区：
文献类型：
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作者：
Yi Xu;M. Kitada;M. Yamaguchi;M. Dezawa;C. Idé
正常成年鼠脊髓中神经祖细胞，特别是巢蛋白+细胞或brdu摄取细胞数量稀少。然而，在本研究中，我们发现脊髓损伤（SCI）后，许多通常静止的细胞在室管膜层和脊髓实质中被激活成为巢蛋白+并进行有丝分裂（BrdU+）。Nestin+细胞和BrdU+细胞多数呈免疫组化GFAP+，部分呈放射状胶质细胞形态，部分参与病变边界的形成。损伤大鼠脊髓组织的培养比完整组织的培养更早产生更多的神经球细胞，这些神经球细胞具有多能性和bfgf反应性。免疫组化分析显示，脊髓损伤后存在大量bFGF+细胞，其数量几乎是完整脊髓的15倍。脊髓损伤后bFGF的增加可能激活静止的祖细胞，从而促进细胞增殖。最后，巢蛋白启动子绿色荧光蛋白（GFP）转基因小鼠的脊髓损伤显示出在损伤脊髓中诱导的祖细胞的广泛增殖。这些转基因小鼠损伤脊髓组织的培养提供了直接证据，证明sci激活的巢蛋白+细胞可以产生神经球。因此，如果对这些祖细胞进行适当的操作，脊髓损伤后bFGF反应性祖细胞的激活和伴随的bFGF+细胞数量的增加可能对脊髓修复有益。
The number of neural progenitor cells, especially nestin+cells or BrdU-uptake cells is sparse in the normal adult rodent spinal cord. However, in the present study, we show that after spinal cord injury (SCI), many ordinarily quiescent cells were activated to become nestin+and undergo mitosis (BrdU+) in the ependymal layer as well as in the parenchyma of the spinal cord. Nestin+cells and BrdU+cells were in most cases immunohistochemically GFAP+, some of which displayed radial glial cell morphology and partly participated in the border formation of the lesion. The culturing of injured rat spinal cord tissues generated more neurospheres earlier than did the culturing of intact tissues, and these neurosphere cells were multipotent and bFGF-responsive. Immunohistochemical analysis showed that there existed many bFGF+cells after SCI, the number of which were almost 15 times greater than that in an intact spinal cord. Increased bFGF production after SCI might activate quiescent progenitor cells, and thus initiate their cell proliferation. Finally, SCI to the nestin-promoter green fluorescent protein (GFP) transgenic mice showed broad proliferation of progenitor cells that were induced in the injured spinal cord. The culturing of injured spinal cord tissues from these transgenic mice provides direct evidence that neurospheres can be generated by SCI-activated nestin+cells. Thus, the activation of bFGF-responsive progenitor cells and the concomitant increase in the population of bFGF+cells following SCI might be beneficial for spinal cord repair if these progenitor cells are properly manipulated.