Bacteria in solitary confinement.

Bacteria in solitary confinement.
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DOI:
10.1128/jb.02509-14
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发表时间:
2015-02-15
影响因子:
3.2
通讯作者:
Mullineaux CW
Mullineaux CW
中科院分区:
生物学3区
文献类型:
--
作者:
Mullineaux CW

文献摘要

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即使在克隆细菌培养中,单个细菌也可能显示出大量的随机变异,导致在解释来自培养物中数百万细胞的数据时存在陷阱。在本期的《细菌学杂志》中,作为他们对蓝细菌适应性的研究的一部分,Nanatani等人描述了采用巧妙的微流体装置来轻轻地笼住单个细胞(J Bacteriol 197:676-687,2015,http://dx. doi. org/10. 1128/JB. 02276 - 14)。该设备是一个受欢迎的工具包,可用于探测单个细胞对环境线索的反应。
Even in clonal bacterial cultures, individual bacteria can show substantial stochastic variation, leading to pitfalls in the interpretation of data derived from millions of cells in a culture. In this issue of the Journal of Bacteriology, as part of their study on osmoadaptation in a cyanobacterium, Nanatani et al. describe employing an ingenious microfluidic device that gently cages individual cells (J Bacteriol 197:676–687, 2015, http://dx.doi.org/10.1128/JB.02276-14). The device is a welcome addition to the toolkit available to probe the responses of individual cells to environmental cues.