Primary structure of chum salmon prolactins: occurrence of highly conserved regions.
Primary structure of chum salmon prolactins: occurrence of highly conserved regions.
复制标题
鲑鱼催乳素的一级结构：高度保守区域的出现。
DOI：
10.1016/0003-9861(86)90621-1
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发表时间：
1986
影响因子：
3.9
通讯作者：
H. Kawauchi
中科院分区：
文献类型：
--
作者：
A. Yasuda;H. Itoh;H. Kawauchi
鲑鱼（Oncorhynchus keta）垂体催乳素的完整氨基酸序列已确定。鲑鱼催乳素包含两种变体，I 和 II，通过反相高效液相色谱分离。每个变体都被还原、S-羧甲基化，然后用溴化氰和酶裂解。通过反相高效液相色谱以及凝胶过滤分离所得片段，并通过丹酰基-埃德曼法进行序列分析。两种变体均含有 187 个氨基酸残基，在残基 46-160 和 177-187 处具有两个二硫键，在哺乳动物催乳素的 N 末端部分缺乏连接，并且彼此之间的不同之处在于仅替换了四个氨基酸残基。鲑鱼催乳素 (sPRL) 与绵羊催乳素有 31% 的序列同一性。此外，四个限制区域，即 sPRL (3-21)、(46-60)、(68-83) 和 (160-178)，涵盖了硬骨鱼和哺乳动物激素之间的这种显着保守性，其同一性分别为 47%、87%、62% 和 68%。这些遥远的系统发育物种之间如此显着的同一性强烈表明这些区域可能负责催乳素的生物活性。
The complete amino acid sequence of prolactin from the pituitaries of salmon (Oncorhynchus keta) has been determined. Salmon prolactin comprised two variants, I and II, which were separated by reverse-phase high-performance liquid chromatography. Each variant was reduced,S-carboxymethylated, and then cleaved with cyanogen bromide and enzymes. The resulting fragments were separated by reverse-phase high-performance liquid chromatography, as well as gel filtration, and subjected to sequence analysis by the dansyl-Edman method. Both variants contain 187 amino acid residues with two disulfide linkages at residues 46–160 and 177–187, lack a linkage in the N-terminal portion of mammalian prolactins, and differ from each other by the replacement of only four amino acid residues. Salmon prolactin (sPRL) shows 31% sequence identity with ovine prolactin. Moreover, four restricted regions, i.e., sPRL (3–21), (46–60), (68–83), and (160–178), encompass this significant conservatism between the teleost and the mammalian hormone, with identities of 47, 87, 62, and 68%, respectively. Such considerable identity between these distant phylogenic species strongly suggests that these regions may be responsible for the biological activity of prolactin.