Gellable silk fibroin-polyethylene sponge for hemostasis
Gellable silk fibroin-polyethylene sponge for hemostasis
复制标题
止血用可凝胶丝素蛋白聚乙烯海绵
DOI：
10.1080/21691401.2019.1699805
复制
发表时间：
2020-01-01
影响因子：
5.8
通讯作者：
Wang, XiaoQin
中科院分区：
文献类型：
--
作者：
Wei, Wei;Liu, Jian;Wang, XiaoQin
传统的止血材料止血速度慢，生物相容性差。本文报道了丝素蛋白（SF）的止血性能及其机理。通过将SF和聚乙二醇（PEG，1500 Da）混合，然后将混合溶液冻干，制备可被血液溶解并变为凝胶形式的SF-PEG海绵。使用兔肝创伤模型对SF-PEG海绵、SF海绵（无PEG）对照样品和市售止血材料明胶海绵进行止血试验。结果显示，SF在止血时间（136.17 +/- 62.27 s和249.83 +/- 29.18 s）和失血量（2.16 +/- 1.27 g vs. 4.97 +/- 1.44 g）方面上级明胶海绵。此外，体外实验表明SF-PEG溶胶-凝胶转变促进血小板粘附和聚集，以及血小板-纤维蛋白原相互作用。因此，除了由于PEG诱导的SF快速凝胶化导致的出血端口的物理阻塞之外，SF还可能对血液凝固过程产生影响，这是以前没有报道的现象。总之，SF是一种新型止血材料，可能能够满足各种临床应用中的速度、效率和生物安全性要求。
Traditional haemostatic materials generally have slow hemostasis rate and poor biocompatibility. This paper reports on the haemostatic properties and mechanism of silk fibroin (SF). SF-PEG sponge that could be solubilised and changed to gel form by blood was fabricated through mixing SF and polyethylene glycol (PEG, 1500 Da) followed by lyophilisation of the mixed solution. SF-PEG sponge, together with control samples of SF sponge (no PEG) and a commercially available haemostatic material, gelatine sponge, were subjected to the hemostasis tests using a liver trauma model of rabbit. The results showed that SF was superior to gelatine sponge in hemostasis time (136.17 +/- 62.27 s and 249.83 +/- 29.18 s) and blood loss (2.16 +/- 1.27 g vs. 4.97 +/- 1.44 g). Furthermore, in vitro experiments indicated SF-PEG sol-gel transition promoted platelet adhesion and aggregation, as well as platelet-fibrinogen interaction. Therefore, except for the physical blocking of bleeding port due to PEG-induced SF fast gelation, SF might also have an impact on blood coagulation process, a phenomenon that has not been reported before. In conclusion, SF is a new type of haemostatic material that might be able to meet the requirements of speed, efficiency and biosafety in a variety of clinical applications.