Development of an Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry Method To Determine Inorganic Arsenic in Liver from Chickens Treated with Roxarsone
Development of an Ion Chromatography-Inductively Coupled Plasma-Mass Spectrometry Method To Determine Inorganic Arsenic in Liver from Chickens Treated with Roxarsone
复制标题
DOI：
10.1021/jf302366a
复制
发表时间：
2012-09-19
影响因子：
6.1
通讯作者：
Carson, Mary C.
中科院分区：
文献类型：
--
作者：
Conklin, Sean D.;Shockey, Nohora;Carson, Mary C.
Roxarsone，（4-羟基-3-硝基苯基）胂酸，是一种含砷化合物，自20世纪40年代以来已被批准作为家禽和猪的饲料添加剂;然而，关于可食用组织中存在的残留砷物质的信息很少。建立了一种新的方法提取和测定鸡肝中砷的形态。强碱性溶液溶解肝脏，超滤去除大分子和颗粒物质。离子色谱法分离提取物中的物种[亚砷酸盐、砷酸盐、单甲基胂酸、二甲基胂酸、（4-羟基-3-氨基苯基）胂酸、（4-羟基-3-乙酰氨基苯基）胂酸和洛克沙胂]，然后用电感耦合等离子体质谱法检测。萃取将大部分亚砷酸盐氧化为砷酸盐。对于2 μ g kg（-1）及以上的强化浓度，回收率范围为70 - 120%，相对标准偏差为7 - 34%。我们检测洛克沙胂，其3-氨基和3-乙酰氨基代谢产物，无机砷，和其他未知的砷物种从洛克沙胂处理的鸡的肝脏。发起实验室和第二个实验室都验证了该方法。
Roxarsone, (4-hydroxy-3-nitrophenyl)arsonic acid, is an arsenic-containing compound that has been approved as a feed additive for poultry and swine since the 1940s; however, little information is available regarding residual arsenic species present in edible tissues. We developed a novel method for the extraction and quantification of arsenic species in chicken liver. A strongly basic solution solubilized the liver, and ultrafiltration removed macromolecules and particulate material. Ion chromatography separated the species [arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, (4-hydroxy-3-aminophenyl)arsonic acid, (4-hydroxy-3-acetaminophenyl)arsonic acid, and roxarsone] in the extracts, which were then detected by inductively coupled plasma-mass spectrometry. The extraction oxidized most arsenite to arsenate. For fortification concentrations at 2 mu g kg(-1) and above, recoveries ranged from 70 to 120%, with relative standard deviations from 7 to 34%. We detected roxarsone, its 3-amino and 3-acetamido metabolites, inorganic arsenic, and additional unknown arsenic species in livers from roxarsone-treated chickens. Both the originating laboratory and a second laboratory validated the method.