Lymphocyte granule-mediated cytolysis requires serine protease activity.
Lymphocyte granule-mediated cytolysis requires serine protease activity.
复制标题
淋巴细胞颗粒介导的细胞溶解需要丝氨酸蛋白酶活性。
DOI：
10.1016/0006-291x(87)90490-6
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Powers,JC
中科院分区：
文献类型：
--
作者：
Hudig,D;Gregg,NJ;Kam,CM;Powers,JC
我们发现从细胞溶解淋巴细胞中分离的颗粒中存在凝乳胰蛋白酶样和胰蛋白酶样蛋白酶，这些颗粒分别具有水解肽底物z - ph - leu - ph - sbzl和Z-Ala-Gly-Arg-SBzl的能力。我们报道蛋白酶抑制剂可以取消或延迟颗粒介导的细胞溶解。两种基于机制的异香豆素丝氨酸蛋白酶抑制剂和z - gly - leu - ph - ch2cl完全取消了颗粒细胞溶解。利马豆和大豆胰蛋白酶抑制剂和凝乳抑素延缓但不阻止这种细胞溶解。这些数据代表了强大的异香豆素抑制剂作为生物探针的首次使用，并表明淋巴细胞丝氨酸蛋白酶参与颗粒细胞溶解过程。
We show that chymotrypsin-like, as well as trypsin-like, proteases are in granules isolated from cytolytic lymphocytes by the capacity of the granules to hydrolyze the peptide substrates Z-Phe-Leu-Phe-SBzl and Z-Ala-Gly-Arg-SBzl, respectively. We report protease inhibitors that can abrogate or delay granule-mediated cytolysis. Two mechanism-based isocoumarin serine protease inhibitors and Z-Gly-Leu-Phe-CH2Cl completely abrogated granule cytolysis. Lima bean and soybean trypsin inhibitors and chymostatin delayed but did not prevent this cytolysis. These data represent the first use of the powerful isocoumarin inhibitors as biological probes and indicate that lymphocyte serine proteases participate in the granule cytolytic process.