Fern gametophytes of Angiopteris lygodiifolia and Osmunda japonica harbor diverse Mucoromycotina fungi
Fern gametophytes of Angiopteris lygodiifolia and Osmunda japonica harbor diverse Mucoromycotina fungi
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DOI：
10.1007/s10265-019-01121-x
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发表时间：
2019-09-01
影响因子：
2.8
通讯作者：
Imaichi, Ryoko
中科院分区：
文献类型：
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作者：
Ogura-Tsujita, Yuki;Yamamoto, Kohei;Imaichi, Ryoko
植物与真菌之间的菌根共生现象普遍存在，并在植物陆地化和多样化中发挥着重要作用。尽管丛枝菌根（AM）与球囊霉亚门真菌的共生体长期以来被认为是古老而广泛的共生体，但最近的研究表明，毛霉亚门真菌也是祖先共生体，因此有望与许多陆地植物共存。为了探讨是否毛霉亚门定居蕨类植物配子体，我们进行真菌协会与配子体的两个远亲蕨类植物，观音座莲（Marattiales）和薇菜（薇菜目），分子分析。还进行了来自细胞内菌丝卷曲的直接PCR扩增。我们在A.海金沙O. japonica的配子体，分别占配子体的41%（7/17）和50%（49/98），并将它们归入内殖子目的10个操作分类单位。此外，我们使用AM真菌特异性引物，并检测Glomeromycotina序列在所有个人检查。结果表明，球囊菌亚门和毛霉亚门同时在蕨类植物配子体中定殖。我们发现，毛霉亚门存在于Marratiales和Osmundales的蕨类植物配子体中，这意味着多种蕨类植物类群具有毛霉亚门协会。
Mycorrhizal symbiosis between plants and fungi is ubiquitous, and has been played key roles in plant terrestrialization and diversification. Although arbuscular mycorrhizal (AM) symbioses with Glomeromycotina fungi have long been recognized as both ancient and widespread symbionts, recent studies showed that Mucoromycotina fungi were also ancestral symbionts and would thus be expected to co-exist with many land plants. To explore whether Mucoromycotina colonize fern gametophytes, we subjected fungal associations with gametophytes of two distantly related ferns, Angiopteris lygodiifolia (Marattiales) and Osmunda japonica (Osmundales), to molecular analysis. Direct PCR amplification from intracellular hyphal coils was also performed. We detected Mucoromycotina sequences in the gametophytes of A. lygodiifolia and O. japonica at rates of 41% (7/17) and 50% (49/98) of gametophytes, respectively, and assigned them to 10 operational taxonomic units of Endogonales lineages. In addition, we used AM fungal-specific primers and detected Glomeromycotina sequences in all individuals examined. The results suggest that Glomeromycotina and Mucoromycotina colonized fern gametophytes simultaneously. We found that Mucoromycotina were present in fern gametophytes of Marratiales and Osmundales, which implies that a variety of fern taxa have Mucoromycotina associations.