Occlusal disharmony attenuates glucocorticoid negative feedback in aged SAMP8 mice
Occlusal disharmony attenuates glucocorticoid negative feedback in aged SAMP8 mice
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DOI：
10.1016/j.neulet.2007.09.020
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发表时间：
2007-11-05
影响因子：
2.5
通讯作者：
Onozuka, Minoru
中科院分区：
文献类型：
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作者：
Ichihashi, Yukiko;Arakawa, Yoko;Onozuka, Minoru
为了评估咬合不和谐导致认知功能受损的机制，我们使用行为、免疫组织化学和原位杂交技术检查了咬合条件对空间表现和海马糖皮质激素受体（GR）和糖皮质激素受体信使核糖核酸（GRmRNA）表达的影响。与年龄匹配的对照小鼠相比，老年咬合小鼠在水迷宫测试中的学习能力明显受损。对照组和咬合饲养的中青年小鼠之间没有差异。此外，免疫组织化学和原位杂交分析表明，咬伤条件增强了海马 GR 和 GRmRNA 表达与年龄相关的下降。特别是，与年龄匹配的对照小鼠相比，老年咬合小鼠的 GR 和 GRmRNA 表达显着降低。这些发现表明，老年 SAMP8 小鼠的咬伤情况会降低 GR 和 GRmRNA，从而损害下丘脑-垂体-肾上腺反馈抑制，从而导致记忆缺陷。 (C) 2007 Elsevier Ireland Ltd. 保留所有权利。
To evaluate the mechanism underlying impaired cognitive function due to occlusal disharmony, we examined the effect of the bite-raised condition on spatial performance and hippocampal expression of glucocorticoid receptors (GR) and glucocorticoid receptor messenger ribonucleic acid (GRmRNA) using behavioral, immunohistochemical, and in situ hybridization techniques. Learning ability in the water maze test was significantly impaired in aged bite-raised mice compared with age-matched control mice. There was no difference between control and bite-raised young and middle-aged mice. Also, immunohistochemical and in situ hybridization analysis showed that the bite-raised condition enhanced the age-related decrease in GR and GRmRNA expression in the hippocampus. In particular, GR and GRmRNA expressions were significantly decreased in aged bite-raised mice compared to age-matched control mice. These findings suggest that the bite-raised condition in aged SAMP8 mice decreases GR and GRmRNA, which impairs the hypothalamic-pituitary-adrenal feedback inhibition, thereby leading to memory deficits. (C) 2007 Elsevier Ireland Ltd. All rights reserved.