Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy
Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy
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DOI：
10.1063/1.4934874
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发表时间：
2015-10
影响因子：
4
通讯作者：
Ryosuke Takahashi;T. Okajima
中科院分区：
文献类型：
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作者：
Ryosuke Takahashi;T. Okajima
我们提出了多频力调制原子力显微镜（AFM）映射的复杂的剪切模量G* 的活细胞作为频率的函数在50-500 Hz的范围内，在相同的测量时间作为单频力调制测量。AFM技术使我们能够重建图像映射的流变参数，表现出频率依赖的幂律行为相对于G*。这些定量流变学测量揭示了单个细胞在此频率范围内G* 的大的空间变化。此外，我们发现幂律流变参数的重建图像与力曲线或单频力调制测量中获得的图像有很大不同。这表明，前者提供的信息，细胞的细胞内的机械结构，通常不能解决与传统的力测量方法。
We present multi-frequency force modulation atomic force microscopy (AFM) for mapping the complex shear modulus G* of living cells as a function of frequency over the range of 50–500 Hz in the same measurement time as the single-frequency force modulation measurement. The AFM technique enables us to reconstruct image maps of rheological parameters, which exhibit a frequency-dependent power-law behavior with respect to G*. These quantitative rheological measurements reveal a large spatial variation in G* in this frequency range for single cells. Moreover, we find that the reconstructed images of the power-law rheological parameters are much different from those obtained in force-curve or single-frequency force modulation measurements. This indicates that the former provide information about intracellular mechanical structures of the cells that are usually not resolved with the conventional force measurement methods.