Inhibition of PRL-3 gene expression in gastric cancer cell line SGC7901 via microRNA suppressed reduces peritoneal metastasis
Inhibition of PRL-3 gene expression in gastric cancer cell line SGC7901 via microRNA suppressed reduces peritoneal metastasis
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DOI：
10.1016/j.bbrc.2006.07.043
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发表时间：
2006-09-15
影响因子：
3.1
通讯作者：
Ma, Jinping
中科院分区：
文献类型：
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作者：
Li, Zhengrong;Zhan, Wenhua;Ma, Jinping
PRL-3是一种蛋白酪氨酸磷酸酶，其高表达与胃癌淋巴结转移有关。本研究旨在探讨PRL-3表达与胃癌腹膜转移的关系。我们利用基于小鼠miR-155序列的人工miRNA（pCMV-PRL-3 miRNA），在mRNA和蛋白水平上有效地沉默胃癌细胞SGC 7901中PRL-3靶基因的表达。在体外实验中，pCMV-PRL 3 miRNA能显著抑制胃癌细胞的侵袭和迁移，且与细胞增殖无关。在体内，PRL-3基因敲低可有效抑制裸鼠腹膜转移瘤的生长，改善其预后。因此，我们认为，人工miRNA可以抑制PRL-3的表达，PRL-3可能成为胃癌腹膜转移的潜在治疗靶点。(c)2006年爱思唯尔公司All rights reserved.
High expression of PRL-3, a protein tyrosine phosphatase, is proved to be associated with lymph node metastasis in gastric carcinoma from previous studies. In this paper, we examined the relationship between PRL-3 expression and peritoneal metastasis in gastric carcinoma. We applied the artificial miRNA (pCMV-PRL3miRNA), which is based on the murine miR-155 sequence, to efficiently silence the target gene expression of PRL-3 in SGC7901 gastric cancer cells at both mRNA and protein levels. Then we observed that, in vitro, pCMV-PRL3miRNA significantly depressed the SGC7901 cell invasion and migration independent of cellular proliferation. In vivo, PRL-3 knockdown effectively suppressed the growth of peritoneal metastases and improved the prognosis in nude mice. Therefore, we concluded that artificial miRNA can depress the expression of PRL-3, and that PRL-3 might be a potential therapeutic target for gastric cancer peritoneal metastasis. (c) 2006 Elsevier Inc. All rights reserved.