Inverse changes in L1 retrotransposons between blood and brain in major depressive disorder.
Inverse changes in L1 retrotransposons between blood and brain in major depressive disorder.
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重度抑郁症患者血液和大脑之间 L1 逆转录转座子的反向变化
DOI：
10.1038/srep37530
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发表时间：
2016-11-22
影响因子：
4.6
通讯作者：
Xu Q
中科院分区：
文献类型：
--
作者：
Liu S;Du T;Liu Z;Shen Y;Xiu J;Xu Q
长穿插核元件-1 （LINE-1或L1）是一种逆转录转座子，占人类和小鼠基因组的17%，已发现与几种类型的神经系统疾病有关。先前的死后脑研究表明，精神分裂症患者的前额叶皮层L1拷贝数增加。然而，L1反转录转位是否在重度抑郁症（MDD）中发生类似尚不清楚。本研究采用定量PCR分析MDD患者（n = 105）和健康对照（n = 105）外周血中L1拷贝数。结果显示，MDD患者的L1拷贝数增加可能与低甲基化有关。此外，在慢性不可预测轻度应激（CUMS）小鼠抑郁模型中，我们测量了小鼠外周血和5个脑区（前额皮质、海马、杏仁核、伏隔核和室旁下丘脑核）L1拷贝数。有趣的是，应激小鼠血液中L1拷贝数增加，前额叶皮层L1拷贝数减少，而大脑其他区域没有变化。我们的研究结果表明L1的改变可能与MDD的病理生理有关，但MDD中L1逆转录功能障碍的生物学机制仍有待进一步研究。
Long interspersed nuclear element-1 (LINE-1 or L1) is a type of retrotransposons comprising 17% of the human and mouse genome, and has been found to be associated with several types of neurological disorders. Previous post-mortem brain studies reveal increased L1 copy number in the prefrontal cortex from schizophrenia patients. However, whether L1 retrotransposition occurs similarly in major depressive disorder (MDD) is unknown. Here, L1 copy number was measured by quantitative PCR analysis in peripheral blood of MDD patients (n = 105) and healthy controls (n = 105). The results showed that L1 copy number was increased in MDD patients possibly due to its hypomethylation. Furthermore, L1 copy number in peripheral blood and five brain regions (prefrontal cortex, hippocampus, amygdala, nucleus accumbens and paraventricular hypothalamic nucleus) was measured in the chronic unpredictable mild stress (CUMS) model of depression in mice. Intriguingly, increased L1 copy number in blood and the decreased L1 copy number in the prefrontal cortex were observed in stressed mice, while no change was found in other brain regions. Our results suggest that the changes of L1 may be associated with the pathophysiology of MDD, but the biological mechanism behind dysfunction of L1 retrotransposition in MDD remains to be further investigated.
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影响因子：
3.7
作者：
Li W;Jin Y;Prazak L;Hammell M;Dubnau J
通讯作者：
Dubnau J
影响因子：
15.1
作者：
Bollati, V.;Galimberti, D.;Pergoli, L.;Dalla Valle, E.;Barretta, F.;Cortini, F.;Scarpini, E.;Bertazzi, P. A.;Baccarelli, A.
通讯作者：
Baccarelli, A.
影响因子：
64.8
作者：
通讯作者：
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影响因子：
25
作者：
Li, Wanhe;Prazak, Lisa;Chatterjee, Nabanita;Grueninger, Servan;Krug, Lisa;Theodorou, Delphine;Dubnau, Josh
通讯作者：
Dubnau, Josh
影响因子：
82.9
作者：
Kang, Hyo Jung;Voleti, Bhavya;Hajszan, Tibor;Rajkowska, Grazyna;Stockmeier, Craig A.;Licznerski, Pawel;Lepack, Ashley;Majik, Mahesh S.;Jeong, Lak Shin;Banasr, Mounira;Son, Hyeon;Duman, Ronald S.
通讯作者：
Duman, Ronald S.