Characterization of the furin cleavage motif for HIV-1 trimeric envelope glycoprotein by intact LC-MS analysis.
Characterization of the furin cleavage motif for HIV-1 trimeric envelope glycoprotein by intact LC-MS analysis.
复制标题
完整LC-MS分析HIV-1三聚包膜糖蛋白的糠蛋白裂解基序。
DOI：
10.1039/c9an02098e
复制
发表时间：
2020-03-02
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
Generating a soluble and native-like trimeric envelope glycoprotein (Env) with high efficacy as an immunogen has been a major focus for developing an effective vaccine against HIV-1. The Env immunogen is a heavily glycosylated protein composed of 3 identical surface gp120 and gp41 subunits that form into a trimer of heterodimers (3 × 28 N-glycan sites). During Env immunogen production, endogenous furin works to cleave a hexa-arginine motif connecting the gp120 and gp41 subunits, which is needed to ensure proper protein folding and a native-like conformation of Env. Verification of the overall identity and proteolytic cleavage of Env is therefore important for HIV-1 vaccine development and product quality. Herein, we report the first work using LC-MS to (1) achieve fast and accurate intact mass measurement of Env after deglycosylation and (2) confidently identify the furin cleavage sites.
登录
查看更多内容
影响因子：
8.7
作者：
Sanders RW;Moore JP
通讯作者：
Moore JP
影响因子：
6.7
作者：
Sanders RW;Derking R;Cupo A;Julien JP;Yasmeen A;de Val N;Kim HJ;Blattner C;de la Peña AT;Korzun J;Golabek M;de Los Reyes K;Ketas TJ;van Gils MJ;King CR;Wilson IA;Ward AB;Klasse PJ;Moore JP
通讯作者：
Moore JP
影响因子：
82.9
作者：
Xu K;Acharya P;Kong R;Cheng C;Chuang GY;Liu K;Louder MK;O'Dell S;Rawi R;Sastry M;Shen CH;Zhang B;Zhou T;Asokan M;Bailer RT;Chambers M;Chen X;Choi CW;Dandey VP;Doria-Rose NA;Druz A;Eng ET;Farney SK;Foulds KE;Geng H;Georgiev IS;Gorman J;Hill KR;Jafari AJ;Kwon YD;Lai YT;Lemmin T;McKee K;Ohr TY;Ou L;Peng D;Rowshan AP;Sheng Z;Todd JP;Tsybovsky Y;Viox EG;Wang Y;Wei H;Yang Y;Zhou AF;Chen R;Yang L;Scorpio DG;McDermott AB;Shapiro L;Carragher B;Potter CS;Mascola JR;Kwong PD
通讯作者：
Kwong PD
影响因子：
4.8
作者：
AlSalmi, Wadad;Mahalingam, Marthandan;Rao, Venigalla B.
通讯作者：
Rao, Venigalla B.
影响因子：
5.4
作者：
Binley, JM;Sanders, RW;Moore, JP
通讯作者：
Moore, JP