Kinetic bacteriochlorophyll fluorometer
Kinetic bacteriochlorophyll fluorometer
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动力学细菌叶绿素荧光计
DOI：
10.1007/s11120-010-9556-6
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发表时间：
2010
影响因子：
3.7
通讯作者：
P. Maróti
中科院分区：
文献类型：
--
作者：
P. Kocsis;E. Asztalos;Z. Gingl;P. Maróti
以半导体激光器为单一光源的泵浦和探针式荧光仪，用于测定光合作用细菌的完整细胞、发色团和分离反应中心的荧光产率的快速诱导和驰豫。该荧光计的时间分辨率受探测闪光的重复时间限制为20μS。该仪器具有高灵敏度、卓越的性能，可成为一系列要求苛刻的应用的多功能设备。其中一些方法包括：(1)不同细菌菌株和突变体完整细胞的光合作用机构的组织和氧化还原状态；(2)分离反应中心供体和受体侧的电子转移反应。机械、光学、电子和数据处理的紧凑设计使该设备易于用作室外仪器或集成到更大的测量系统中。
A pump and probe fluorometer with a laser diode as single light source has been constructed for measurement of fast induction and relaxation of the fluorescence yield in intact cells, chromatophores and isolated reaction centers of photosynthetic bacteria. The time resolution of the fluorometer is limited by the repetition time of the probing flashes to 20 μs. The apparatus offers high sensitivity, excellent performance and can become a versatile device for a range of demanding applications. Some of them are demonstrated here including fast and easy investigation of the (1) organization and redox state of the photosynthetic apparatus of the intact cells of different bacterial strains and mutants and (2) electron transfer reactions on donor and acceptor sides of isolated reaction centers. The compact design of the mechanics, optics, electronics, and data processing makes the device easy to use as outdoor instrument or to integrate into larger measuring systems.
光谱沉默光诱导光合反应中心的构象变化
DOI：
--
发表时间：
2008
期刊：
FEBS Lett. 582
影响因子：
--
作者：
L. Nagy;P. Maroti;M. Terazima
通讯作者：
M. Terazima