THE NUCLEUS BASALIS-MAGNOCELLULARIS - THE ORIGIN OF A CHOLINERGIC PROJECTION TO THE NEOCORTEX OF THE RAT
THE NUCLEUS BASALIS-MAGNOCELLULARIS - THE ORIGIN OF A CHOLINERGIC PROJECTION TO THE NEOCORTEX OF THE RAT
复制标题
DOI：
10.1016/0306-4522(80)90195-5
复制
发表时间：
1980-01-01
期刊：
影响因子：
3.3
通讯作者：
FIBIGER, HC
中科院分区：
文献类型：
--
作者：
LEHMANN, J;NAGY, JI;FIBIGER, HC
通过顺行和逆行方法在大鼠中鉴定新皮质胆碱能传入投射起源的细胞。将辣根过氧化物酶注射到苍白球腹内侧末端新皮质标记的富含乙酰胆碱酯酶 [EC 3.1.1.7] 的神经元中。这组神经元在皮质消融后经历了逆行性变。发生细胞耗竭的区域也显示胆碱乙酰转移酶和乙酰胆碱酯酶的活性显着降低。仅限于苍白球内侧部分的离散电解和红藻氨酸损伤导致新皮质胆碱乙酰转移酶和乙酰胆碱酯酶的显着消耗。该细胞组尾部的半横切不会导致这种消耗。位于苍白球腹内侧末端的富含乙酰胆碱酯酶的神经元可能构成向新皮质的主要皮质下胆碱能投射的起源。讨论了用氟磷酸二异丙酯预处理的动物中乙酰胆碱酯酶组织化学在鉴定胆碱能神经元中的效用。预处理后 4-8 小时的高乙酰胆碱酯酶活性是鉴定胆碱能核周的必要但不充分的标准。这些神经元似乎与灵长类动物无名质的基底核同源，在大鼠中被称为大细胞基底核。没有获得证据来支持对角带核投射到新皮质的假设。在大细胞基底核、斜带核和内侧隔核的假定胆碱能核周体中观察到大小和乙酰胆碱酯酶活性的惊人相似性。新皮质的红藻氨酸损伤对神经元周核产生均匀且完全的破坏，从而降低了新皮质谷氨酸脱羧酶活性，表明新皮质中存在GABA能周核。相同的病变不影响新皮质胆碱乙酰转移酶，表明新皮质中不存在胆碱能核周，这与新皮质中不存在强烈的乙酰胆碱酯酶反应性神经元一致。
Cells of neocortical cholinergic afferent projection origin were identified by anterograde and retrograde methods in the rat. Horseradish peroxidase injected into neocortex labeled large, acetylcholinesterase[EC 3.1.1.7]-rich neurons in the ventromedial extremity of the globus pallidus. This group of neurons underwent retrograde degeneration following cortical ablations. The region in which cell depletion occurred also showed significant decreases in the activities of choline acetyltransferase and acetylcholinesterase. Discrete electrolytic and kainic acid lesions restricted to the medial part of the globus pallidus resulted in significant depletions of neocortical choline acetyltransferase and acetylcholinesterase. Hemitransections caudal to this cell group did not result in such depletions. The acetylcholinesterase-rich neurons lying in the ventromedial extremity of the globus pallidus may constitute the origin of a major subcortical cholinergic projection to the neocortex. The utility of acetylcholinesterase histochemistry in animals pretreated with diisopropylphosphorofluoridate in identifying cholinergic neurons is discussed. High acetylcholinesterase activity 4-8 h after this pretreatment is a necessary but not sufficient criterion for the identification of cholinergic perikarya. The neurons appear to be homologous to the nucleus basalis of the substantia innominata of primates, and are termed nucleus basalis magnocellularis in the rat. No evidence was obtained to support the hypothesis that nucleus of the diagonal band projects to neocortex. Striking similarities in size and acetylcholinesterase activity were observed among the putative cholinergic perikarya of the nucleus basalis magnocellularis, the nucleus of the diagonal band and the medial septal nucleus. Kainic acid lesions of the neocortex produced uniform and complete destruction of neuronal perikarya, which decreased neocortical glutamic acid decarboxylase activity, suggesting that there are GABAergic perikarya in the neocortex. The same lesions did not affect neocortical choline acetyltransferase, suggesting that there are no cholinergic perikarya in the neocortex, which is consistent with the absence of intensely acetylcholinesterase-reactive neurons in the neocortex.