LOW-DENSITY-LIPOPROTEIN INDUCES VASCULAR ADHESION MOLECULE EXPRESSION ON HUMAN ENDOTHELIAL-CELLS
LOW-DENSITY-LIPOPROTEIN INDUCES VASCULAR ADHESION MOLECULE EXPRESSION ON HUMAN ENDOTHELIAL-CELLS
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DOI：
10.1161/01.hyp.25.4.511
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发表时间：
1995-04-01
期刊：
影响因子：
8.3
通讯作者：
LUFT, FC
中科院分区：
文献类型：
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作者：
HALLER, H;SCHAPER, D;LUFT, FC
我们验证了低密度脂蛋白（LDL）及其乙酰化形式影响人内皮细胞血管粘附分子表面表达的假设。采用改良的酶联免疫吸附法，用流式细胞术对培养的内皮细胞进行血管粘附分子表面表达评估。用色谱法测定LDL乙酰化。用U937细胞直接计数评估单核细胞与内皮细胞的粘附。阳性对照肿瘤坏死因子α (10 ng/mL)诱导血管粘附分子的时间依赖性表达(P
We tested the hypothesis that low-density lipoprotein (LDL) and its acetylated form influence surface expression of vascular adhesion molecules on human endothelial cells. Vascular adhesion molecule surface expression was assessed with flow cytometry on cultured endothelial cells with a modified enzyme-linked immunosorbent assay. LDL acetylation was determined by chromatography. Monocyte adhesion to endothelial cells was assessed with U937 cells by direct counting. Tumor necrosis factor-alpha (10 ng/mL), a positive control, induced a time-dependent expression of vascular adhesion molecules (P