Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow Cytometry.
Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow Cytometry.
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通过免疫淘选和低压流式细胞术纯化从分化到成体的视网膜神经节细胞。
DOI：
10.1007/978-1-0716-3409-7_2
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kador,KarlE
中科院分区：
文献类型：
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作者：
Riordan,SeanM;Aladdad,AfnanM;McLoughlin,KiranJ;Kador,KarlE
啮齿动物视网膜神经节细胞（RGC）的分离和培养是研究这一关键细胞类型的功能和细胞反应的关键步骤。用于分离RGC的典型方法包括用靶向RGC特异性蛋白质标记物的抗体进行免疫淘选或磁珠分离。然而，在发育研究中，许多最常见的标记物，如Thy-1，在发育的早期阶段不表达。为了帮助研究这些关键的早期RGCs，我们开发了一种新的方法，该方法利用了在蛋白质BRN 3上带有GFP标签的转基因小鼠和低压荧光激活细胞分选仪（FACS）系统。
The isolation and culturing of rodent retinal ganglion cells (RGC) is a key step in studying the function and cellular response of this crucial cell type. Typical methods used for isolation of RGCs include immunopanning or magnetic bead separation with antibodies targeting RGC specific protein markers. However, in developmental research, many of the most common markers, such as Thy-1, are not expressed in early stages of development. To help study these crucial early stage RGCs, we have developed a novel method that utilizes a transgenic mouse with a GFP tag on the protein BRN3 and a low-pressure fluorescence-activated cell sorter (FACS) system.