Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro.
Growth hormone in the presence of laminin modulates interaction of human thymic epithelial cells and thymocytes in vitro.
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DOI：
10.1186/s40659-016-0097-0
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发表时间：
2016-09-02
影响因子：
6.7
通讯作者：
Smaniotto S
中科院分区：
文献类型：
--
作者：
Lins MP;de Araújo Vieira LF;Rosa AA;Smaniotto S
一些证据表明，激素和神经肽起免疫调节剂的作用。其中，生长激素(GH)作用于胸腺微环境，支持其在胸腺细胞分化中的作用。本研究的目的是评价生长激素在层粘连蛋白存在的情况下对人胸腺细胞和胸腺上皮细胞的影响。GH可增加胸腺细胞在牛血清白蛋白涂层和层粘连蛋白涂层表面的黏附。GH处理组小鼠胸腺细胞膜层粘连蛋白包被层粘连蛋白的迁移细胞数明显高于对照组。在这两个结果中，胸腺细胞上VLA-6的表达是恒定的。此外，GH处理可促进TEC在培养24小时后产生层粘连蛋白。而VLA-6整合素在TEC上的表达无明显变化。TEC/胸腺细胞共培养模型显示，GH可增加双阴性(CD_4、−、CD_8、−)和CD_4~+、CD_8~+单阳性胸腺细胞的绝对数。在双阳性(CD4+CD8+)胸腺细胞中发现细胞数量减少。本研究结果表明，生长激素能够在层粘连蛋白存在的情况下增强人胸腺细胞的迁移能力，并促进胸腺细胞亚群与TEC共培养后的调节。
Several evidences indicate that hormones and neuropeptides function as immunomodulators. Among these, growth hormone (GH) is known to act on the thymic microenvironment, supporting its role in thymocyte differentiation. The aim of this study was to evaluate the effect of GH on human thymocytes and thymic epithelial cells (TEC) in the presence of laminin. GH increased thymocyte adhesion on BSA-coated and further on laminin-coated surfaces. The number of migrating cells in laminin-coated membrane was higher in GH-treated thymocyte group. In both results, VLA-6 expression on thymocytes was constant. Also, treatment with GH enhanced laminin production by TEC after 24 h in culture. However, VLA-6 integrin expression on TEC remained unchanged. Finally, TEC/thymocyte co-culture model demonstrated that GH elevated absolute number of double-negative (CD4−CD8−) and single-positive CD4+ and CD8+ thymocytes. A decrease in cell number was noted in double-positive (CD4+CD8+) thymocytes. The results of this study demonstrate that GH is capable of enhancing the migratory capacity of human thymocytes in the presence of laminin and promotes modulation of thymocyte subsets after co-culture with TEC.
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DOI：
10.1155/1998/89782
发表时间：
1998-01-01
期刊：
Developmental immunology
影响因子：
--
作者：
De Mello-Coelho, V;Savino, W;Dardenne, M
通讯作者：
Dardenne, M
DOI：
10.1111/nyas.12857
发表时间：
2015-01-01
期刊：
NEUROIMMUNOMODULATION IN HEALTH AND DISEASE
影响因子：
--
作者：
Bodart, Gwennaelle;Goffinet, Lindsay;Martens, Henri J.
通讯作者：
Martens, Henri J.
影响因子：
2.7
作者：
Savino, Wilson
通讯作者：
Savino, Wilson
影响因子：
2.4
作者：
Kermani, Hamid;Goffinet, Lindsay;Martens, Henri J.
通讯作者：
Martens, Henri J.
影响因子：
8.7
作者：
Chaudhry MS;Velardi E;Dudakov JA;van den Brink MR
通讯作者：
van den Brink MR