Colloidal bismuth subcitrate (De-Nol) inhibits degradation of gastric mucus by Campylobacter pylori protease.
Colloidal bismuth subcitrate (De-Nol) inhibits degradation of gastric mucus by Campylobacter pylori protease.
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DOI：
10.1111/j.1572-0241.1989.tb02571.x
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发表时间：
1989-05
期刊：
影响因子：
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通讯作者：
J. Sarosiek;J. Bilski;V. Murty;A. Slomiany;B. Slomiany
中科院分区：
文献类型：
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作者：
J. Sarosiek;J. Bilski;V. Murty;A. Slomiany;B. Slomiany
越来越多的人认识到，幽门弯曲杆菌感染可能是胃疾病发病机制中的一个主要因素。在这里，我们提供的证据表明，这种细菌产生的细胞外蛋白酶（导致胃粘液降解）受到抗溃疡剂胶体次柠檬酸铋（CBS; De-Nol）的抑制。本研究以C.从接受胃镜检查的患者的胃窦粘膜活检标本中培养出幽门螺杆菌。将生长的细菌菌落用盐水洗涤，通过灭菌过滤器过滤，透析，并冻干。将粉末用作使用猪胃粘液作为底物的蛋白水解活性测定的酶源。在37 ℃和pH 7.0下获得最佳酶活性。C.的表观Km。胃粘液中pylori蛋白酶为0.71g/L。降解产物的分析表明，蛋白酶引起粘液糖蛋白聚合物的广泛蛋白水解。将CBS引入孵育混合物导致粘液降解速率降低。蛋白水解抑制率与CBS浓度成正比，直至1 × 10（-1）g/L，此时粘液蛋白水解减少37%。测定了C. pylori蛋白酶活性为1.25g/L。结果表明，CBS能够对抗C.幽门。
There is increased awareness that infection with Campylobacter pylori could be a major factor in the pathogenesis of gastric disease. Here, we present evidence that the extracellular protease elaborated by this bacteria, which causes degradation of gastric mucus, is inhibited by an antiulcer agent, colloidal bismuth subcitrate (CBS; De-Nol). The study was conducted with C. pylori cultured from antral mucosal biopsy specimens of patients undergoing gastroscopy. The grown colonies of bacteria were washed with saline, filtered through sterilization filter, dialyzed, and lyophilized. The powder was used as the enzyme source for proteolytic activity assay employing pig gastric mucus as substrate. Optimum enzymatic activity was obtained at 37 degrees C and at pH 7.0. The apparent Km of C. pylori protease with gastric mucus was 0.71 g/L. Analyses of the degradation products indicated that the protease caused extensive proteolysis of mucus glycoprotein polymer. Introduction of CBS to the incubation mixtures led to a reduction of the rate of mucus degradation. The rate of proteolysis inhibition was proportional to CBS concentration up to 1 X 10(-1) g/L, at which point a 37% reduction in mucus proteolysis was obtained. The Km value for proteolytic degradation of mucus by C. pylori protease in the presence of CBS was 1.25 g/L. The results suggest that CBS is capable of counter-acting the proteolysis of the protective gastric mucus layer by C. pylori.