A Comprehensive Mouse Transcriptomic BodyMap across 17 Tissues by RNA-seq.
A Comprehensive Mouse Transcriptomic BodyMap across 17 Tissues by RNA-seq.
复制标题
通过 RNA-seq 绘制 17 个组织的全面小鼠转录体图谱。
DOI：
10.1038/s41598-017-04520-z
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发表时间：
2017-06-23
影响因子：
4.6
通讯作者：
Shi L
中科院分区：
文献类型：
--
作者：
Li B;Qing T;Zhu J;Wen Z;Yu Y;Fukumura R;Zheng Y;Gondo Y;Shi L
小鼠已被广泛用作研究人类疾病和评价药物安全性和有效性的模式生物。许多疾病和药物效应表现出组织特异性，这可能反映在组织特异性基因表达谱上。本研究利用RNA-seq技术构建了6周龄C57BL/6JJcl小鼠17个组织的完整的小鼠转录组BodyMap。我们发现蛋白质编码基因和非编码基因之间存在不同的表达模式。肝脏表达最不复杂的转录组，也就是说，在所有17个组织中肝脏中检测到的基因数量最少，而睾丸和卵巢比其他组织含有更复杂的转录组。我们报告了17个组织中组织特异性基因的综合列表，以及小鼠中4,781个管家基因的列表。此外，我们提出了一个27个一致和高表达基因的列表，可以用作表达谱分析的参考对照。我们的研究提供了一个独特的小鼠基因表达谱资源，有助于进一步的生物医学研究。
The mouse has been widely used as a model organism for studying human diseases and for evaluating drug safety and efficacy. Many diseases and drug effects exhibit tissue specificity that may be reflected by tissue-specific gene-expression profiles. Here we construct a comprehensive mouse transcriptomic BodyMap across 17 tissues of six-weeks old C57BL/6JJcl mice using RNA-seq. We find different expression patterns between protein-coding and non-coding genes. Liver expressed the least complex transcriptomes, that is, the smallest number of genes detected in liver across all 17 tissues, whereas testis and ovary harbor more complex transcriptomes than other tissues. We report a comprehensive list of tissue-specific genes across 17 tissues, along with a list of 4,781 housekeeping genes in mouse. In addition, we propose a list of 27 consistently and highly expressed genes that can be used as reference controls in expression-profiling analysis. Our study provides a unique resource of mouse gene-expression profiles, which is helpful for further biomedical research.