Eilat virus induces both homologous and heterologous interference
Eilat virus induces both homologous and heterologous interference
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DOI：
10.1016/j.virol.2015.05.009
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发表时间：
2015-10-01
期刊：
影响因子：
3.7
通讯作者：
Weaver, Scott C.
中科院分区：
文献类型：
--
作者：
Nasar, Farooq;Erasmus, Jesse H.;Weaver, Scott C.
大多数甲病毒是蚊子传播的，宿主范围广泛，感染许多不同的脊椎动物，包括鸟类、啮齿动物、马科动物和人类。有时，甲病毒会扩散到人群中并引起以衰弱性关节痛或致命性脑炎为特征的疾病。最近，一种独特的甲病毒，埃拉特病毒（EILV），被描述为很容易感染蚊子，但不易感染脊椎动物细胞系。在这里，我们研究了EILV诱导重复感染排除的能力。先前用 EILV 感染 C7/10（白纹伊蚊）细胞会诱导同源和异源干扰，使异源重复感染病毒（SINV、VEEV、EEEV、WEEV 和 CHIKV）的病毒滴度降低约 10-10,000 倍，并将复制动力学延迟 12-48 小时。与体外感染类似，埃及伊蚊体内EILV感染使基孔肯雅病毒的传播延迟了3天。这是蚊子特异性甲病毒在体外和体内诱导异源干扰的第一个证据。 (C) 2015 Elsevier Inc. 版权所有。预订的。
Most alphaviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, and humans. Occasionally, alphaviruses can spill over into the human population and cause disease characterized by debilitating arthralgia or fatal encephalitis. Recently, a unique alphavirus, Eilat virus (EILV), was described that readily infects mosquito but not vertebrate cell lines. Here, we investigated the ability of EILV to induce superinfection exclusion. Prior infection of C7/10 (Aedes albopictus) cells with EILV induced homologous and heterologous interference, reducing the virus titers of heterologous superinfecting viruses (SINV, VEEV, EEEV, WEEV, and CHIKV) by similar to 10-10,000 fold and delaying replication kinetics by 12-48 h. Similar to in vitro infection, prior in vivo EILV infection of Aedes aegypti mosquitoes delayed dissemination of chikungunya virus for 3 days. This is the first evidence of heterologous interference induced by a mosquito-specific alphavirus in vitro and in vivo. (C) 2015 Elsevier Inc. All rights. reserved.