Lophine derivatives as versatile analytical tools.
Lophine derivatives as versatile analytical tools.
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DOI：
10.1002/bmc.226
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发表时间：
2003-03
期刊：
影响因子：
--
通讯作者：
K. Nakashima
中科院分区：
文献类型：
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作者：
K. Nakashima
描述了作为生物医学科学中的通用分析工具的Lophine（2，4，5-三苯基咪唑）衍生物。化学发光（CL）和荧光（FL）性质的洛啡衍生物的第一个演示，包括CL反应机理，取代基的CL产率的影响，FL光谱行为等。接下来，分析应用，如钴（II）和铬（VI）的测定进行了讨论。最后介绍了洛啡碱衍生物作为化学发光和荧光试剂在生物材料中有机物测定中的应用研究。其中，2-（4-肼基羰基苯基）-4，5-二苯基咪唑（HCPI）和4-（4，5-二苯基-1H-咪唑-2-基）苯甲酰氯（DIB-Cl）在高效液相色谱中分别作为脂肪酸、胺和/或酚类的标记试剂具有优异的性能。硼酸衍生物作为CL增强剂的效用也在这篇评论中进行了讨论。
Lophine (2,4,5-triphenylimidazole) derivatives as versatile analytical tools in biomedical sciences are described. Chemiluminescence (CL) and fluorescence (FL) properties of the lophine derivatives are first demonstrated including the CL reaction mechanism, effects of substituents on CL yields, FL spectral behaviors, etc. Next, analytical applications to the determination of metal ions such as cobalt (II) and chromium (VI) are discussed. Finally, the application studies of lophine derivatives as CL and FL reagents for the determination of organic substances in biological materials are presented. Among the derivatives, 2-(4-hydrazinocarbonylphenyl)-4,5-diphenylimidazole (HCPI) and 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) are studied, with their excellent properties as labeling reagents for fatty acids and amines and/or phenols, respectively, in high-performance liquid chromatography. The utility of boronic acid derivatives as CL enhancers is also discussed in this review.