Renaturation and reconstitution of functional holoenzyme from recombinant subunits of casein kinase II expressed as inclusion bodies in E. coli.
Renaturation and reconstitution of functional holoenzyme from recombinant subunits of casein kinase II expressed as inclusion bodies in E. coli.
复制标题
在大肠杆菌中表达为包涵体的酪蛋白激酶 II 重组亚基的功能性全酶的复性和重建。
DOI：
10.1007/978-1-4899-1766-9_7
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Traugh,JA
中科院分区：
文献类型：
--
作者：
Lin,WJ;Jakobi,R;Traugh,JA
酪蛋白激酶II（蛋白激酶CKII）是由两个α和两个β亚基组成的异源四聚体（1，2）。α亚基可以表达为两种不同的基因产物，其以α和/或α′形式存在，这取决于组织和物种的来源。β亚基是高等真核生物中的单基因产物。来自许多不同物种的序列的比较显示α和β亚基是高度保守的（1，3）。异源四聚体是稳定的，并且结构仅在变性条件下可解离。因此，在分子水平上对酶进行完整的物理、化学和功能分析取决于获得能够重组成活性CKII的亚基。这一重组的CKII必须有能力以适当的方式与周围环境相互作用并作出反应。
Casein kinase II (protein kinase CKII) is a heterotetramer consisting of twoαand twoβsubunits (1,2). Theαsubunit can be expressed as two different gene products which are present asαand/or α′, depending on the tissue and species of origin. Theβsubunit is a single gene product in higher eukaryotes. A comparison of the sequences from a number of different species shows theαandβsubunits are highly conserved (1,3). The heterotetramer is stable and the structure is dissociable only under denaturing conditions. Thus, complete physical, chemical and functional analysis of the enzyme at the molecular level is dependent on obtaining subunits capable of reconstituting into active CKII. This reconstituted CKII must have the capacity to interact with and react to the surrounding environment in an appropriate manner.