Evaluation of an ovary-on-a-chip in large mammalian models: Species specificity and influence of follicle isolation status.
Evaluation of an ovary-on-a-chip in large mammalian models: Species specificity and influence of follicle isolation status.
复制标题
大型哺乳动物模型中卵巢芯片的评估：物种特异性和卵泡分离状态的影响。
DOI：
10.1002/term.2623
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发表时间：
2018
影响因子：
3.3
通讯作者：
Demirci,Utkan
中科院分区：
文献类型：
--
作者：
Nagashima,JenniferB;ElAssal,Rami;Songsasen,Nucharin;Demirci,Utkan
从未成熟卵泡中体外培养卵母细胞的能力具有显著的生育力保存潜力;然而，由于复杂的代谢需求和长期培养要求，在大型哺乳动物物种中证明具有挑战性。目前，卵泡孵育是基于手动更换培养基的“静态”系统。尽管传统培养方法具有许多优点，但重现天然微环境并支持大型哺乳动物物种的卵泡存活仍然是一个挑战。在这项研究中，我们利用了一种创新的，动态的微流体系统，以支持在卵巢皮质内封闭或从卵巢皮质分离的家猫和狗卵泡的体外生存。结果表明响应于微流体培养的物种特异性和组织类型特异性差异。家猫但不是狗卵巢皮质组织保持活力下流动类似于传统的琼脂糖凝胶对照。腔前阶段分离的卵泡从这两个物种中生长最有利的传统藻酸盐珠培养，但总体而言，没有影响的卵泡发育或卵母细胞健康标志物的表达的培养系统。该系统代表了对大型哺乳动物物种（例如，猫和狗），其具有用于生育力保存、生殖毒理学和濒危哺乳动物保护工作的潜在应用。
The ability to grow oocytes from immature ovarian follicles in vitro has significant potential for fertility preservation; yet, it has proved challenging in large mammalian species due to the complex metabolic needs and long‐term culture requirements. Currently, follicular incubations are based on a “static” system with manual exchange of medium. Despite the numerous advantages of conventional culturing approaches, recapitulating the native microenvironment and supporting the survival of ovarian follicles from large mammalian species still represent challenges. In this study, we utilized an innovative, dynamic microfluidic system to support the in vitro survival of domestic cat and dog follicles enclosed within the ovarian cortex or isolated from ovarian cortex. Results indicate both species‐specific and tissue type‐specific differences in response to microfluidic culture. Domestic cat but not dog ovarian cortical tissues maintained viability under flow similar to conventional agarose gel controls. Preantral stage isolated follicles from both species that grew most favourably in conventional alginate bead culture, but overall, there was no influence of culture system on expression of follicle development or oocyte health markers. This system represents an important exploration toward the development of an improved ovarian in vitro culture system of large mammalian species (e.g., cats and dogs), which has potential applications for fertility preservation, reproductive toxicology, and endangered mammal conservation efforts.