RAPID INDUCTION OF SELECTIVE TRANSCRIPTION BY AUXINS
RAPID INDUCTION OF SELECTIVE TRANSCRIPTION BY AUXINS
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DOI：
10.1128/mcb.5.6.1197
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
GUILFOYLE, TJ
中科院分区：
文献类型：
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作者：
HAGEN, G;GUILFOYLE, TJ
从用2，4-D处理的离体大豆[Glycine max]幼芽中分离的细胞核在4种生长素调节的基因或DNA序列的转录中具有活性，这在以前已经描述过（哈根，Kleinschleman和Guilfoyle，1984）。生长素反应序列的转录速率是10- 100倍，从生长素处理的胚芽分离的细胞核比未处理的胚芽。用完整大豆幼苗和下胚轴切片的下胚轴，以及用2，4-D处理的绿色菜豆[Phaseolus vulgaris]和绿豆[Vigna radiata]幼芽也观察到转录反应。其它生长素，包括2，4，5-T，α-萘乙酸和IAA也能诱导转录反应。增加转录率后5分钟内观察到应用生长素切除幼芽，和半最大的最大转录率达到应用生长素后15分钟。低至10-7至10-8 M2，4-D诱导转录反应，但在10-3 M2，4-D达到最大转录率。用蛋白质合成抑制剂放线菌酮进行短暂处理并不抑制生长素对转录的诱导。生长素调控的基因表达处于快速转录控制之下。
Nuclei isolated from excised soybean [Glycine max] plumules that were treated with 2,4-D were active in transcription of 4 auxin-regulated genes or DNA sequences, which have been described previously (Hagen, Kleinschmidt and Guilfoyle, 1984). The rates of transcription of the auxin-responsive sequences were 10- to 100-fold greater with nuclei isolated from auxin-treated plumules than with those from untreated plumules. The transcriptional response was also observed with hypocotyls of intact soybean seedlings and hypocotyl sections, as well as with green bean [Phaseolus vulgaris] and mung bean [Vigna radiata] plumules that were treated with 2,4-D. Other auxins, including 2,4,5-T, .alpha.-naphthaleneacetic acid, and IAA also induced the transcriptional response. Increased transcription rates were observed within 5 min after application of auxins to excised plumules, and half-maximal to maximal transcription rates were achieved by 15 min after application of auxins. As little as 10-7 to 10-8 M 2,4-D induced a transcriptional response, but maximal transcription rates were achieved at 10-3 M 2,4-D. Brief treatment with the protein synthesis inhibitor cycloheximide did not inhibit the induction of transcription by auxins. Auxin-regulated gene expression is under rapid transcriptional control.