Stage‐specific expression of a homeo box‐containing gene in the non‐segmented sea urchin embryo.
Stage‐specific expression of a homeo box‐containing gene in the non‐segmented sea urchin embryo.
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含有同源盒的基因在非节段海胆胚胎中的阶段特异性表达。
DOI：
10.1002/j.1460-2075.1986.tb04305.x
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
Tom Humphreys
中科院分区：
文献类型：
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作者：
G. Dolecki;Surawit Wannakrairojl;Richard Lum;Gordon Wang;Henry D. Riley;Ruben Carlos;Allan Wang;Tom Humphreys
果蝇同源盒DNA探针与来自夏威夷海胆Tripneustes gratilla的基因组DNA的Southern转移杂交显示，海胆基因组包含至少5个同源盒。从几个人的DNA检查表明，这些同源盒侧翼的序列表现出很少的限制性片段长度多态性，表明它们比大多数海胆DNA更高度保守。一个T.对与果蝇同源盒探针杂交的gratilla基因组DNA文库进行了鉴定，并选择了一个在胚胎发育过程中转录的基因进行了进一步的研究。Southern转移杂交显示克隆的基因是单拷贝的。海胆基因的DNA测序确定了一个与果蝇同源盒探针70 - 73%同源的同源盒，以及一个与探针编码的同源结构域78 - 88%同源的编码同源结构域。将含有海胆同源盒基因的DNA探针与胚胎RNA的北方转移杂交，证明该基因产生6.9 kb和7.7 kb的两种转录本。转录本首先在囊胚期积累，并在原肠胚期增加到最高水平，然后在pluteus阶段大量减少。
Hybridization of Drosophila homeo box DNA probes to Southern transfers of genomic DNA from the Hawaiian sea urchin Tripneustes gratilla has revealed that the sea urchin genome contains at least five homeo boxes. Examination of the DNA from several individuals shows that the sequences flanking these homeo boxes exhibit little restriction fragment length polymorphism, indicating they are more highly conserved than the majority of sea urchin DNA. Several clones in a T. gratilla genomic DNA library which hybridized with Drosophila homeo box probes were identified, and one found to be transcribed during embryogenesis was selected for further study. Southern transfer hybridizations showed the cloned gene to be single‐copy. DNA sequencing of the sea urchin gene defined a homeo box 70‐73% homologous to the Drosophila homeo box probes and an encoded homeo domain 78‐88% homologous to those encoded by the probes. Hybridization of DNA probes from the sea urchin homeo box‐containing gene to Northern transfers of embryonic RNA demonstrated that the gene produces two transcripts of 6.9 kb and 7.7 kb. Transcripts first accumulate at blastula stage and increase to a maximum level at gastrula stage before decreasing considerably in abundance by pluteus stage.