SAMMA, a mandelic acid condensation polymer, inhibits dendritic cell-mediated HIV transmission
SAMMA, a mandelic acid condensation polymer, inhibits dendritic cell-mediated HIV transmission
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DOI：
10.1016/j.febslet.2007.08.048
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发表时间：
2007-10-02
期刊：
影响因子：
3.5
通讯作者：
Klotman, Mary E.
中科院分区：
文献类型：
--
作者：
Chang, Theresa L.;Teleshova, Natalia;Klotman, Mary E.
SAMMA是一种扁桃酸缩合聚合物，对包括人类免疫缺陷病毒（HIV）在内的几种性传播病原体具有广泛的抗菌活性。在这里，我们证明了SAMMA抑制了树突状细胞（dc）的HIV传播，树突状细胞是原发性感染的第一个靶细胞之一。当SAMMA与靶细胞共培养时，达到最大的抑制效果。SAMMA对dc介导的HIV传播的抑制作用不是由于细胞毒性。对dc相关HIV p24抗原水平的分析显示，当病毒与SAMMA化合物一起预孵卵时，SAMMA可以阻止dc内化HIV。相比之下，用SAMMA对dc进行预孵育，然后冲洗，不影响细胞相关HIV p24抗原的数量。此外，SAMMA阻断HIV糖蛋白介导的细胞-细胞融合。这项研究表明SAMMA通过多种机制预防HIV感染。(c) 2007年欧洲生化学会联合会。Elsevier B.V.版权所有。
SAMMA, a mandelic acid condensation polymer, exhibits a broad antimicrobial activity against several sexually transmitted pathogens including human immunodeficiency virus (HIV). Here we demonstrated that SAMMA suppressed HIV transmission by dendritic cells (DCs), one of the first target cells for primary infection. The greatest inhibitory effect was achieved when SAMMA was present during the co-culture with target cells. The inhibitory effect of SAMMA on DC-mediated HIV transmission was not due to cytotoxicity. Analysis of the level of DC-associated HIV p24 antigen revealed that SAMMA prevented HIV internalization by DCs when the virus was pre-incubated with the compound. In contrast, pre-incubation of DCs with SAMMA followed by wash-off did not affect the amount of cell-associated HIV p24 antigen. In addition, SAMMA blocked HIV glycoprotein-mediated cell-cell fusion. This study suggests that SAMMA prevents HIV infection through multiple mechanisms. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.