Membrane localization and dynamics of Nile Red: Effect of cholesterol
Membrane localization and dynamics of Nile Red: Effect of cholesterol
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DOI：
10.1016/j.bbamem.2006.07.010
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发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Chattopadhyay, Amitabha
中科院分区：
文献类型：
--
作者：
Mukherjee, Soumi;Raghuraman, H.;Chattopadhyay, Amitabha
组织和动力学的疏水荧光探针尼罗红纳入DOPC囊泡含有不同量的胆固醇已被监测，利用基于荧光的方法，其中包括红边激发位移（REES）的方法和视差的深度测定方法。我们的研究结果表明，尼罗红的荧光发射最大值，强度，偏振，和寿命的变化与胆固醇含量的膜。有趣的是，尼罗红表现出显著的REES，与胆固醇的存在无关。这表明尼罗红定位于膜中运动受限的环境中。这是支持的膜渗透深度的尼罗红的视差法，指出了膜界面定位的尼罗红的分析。这些结果可能是有用的，在分析膜的组织和异质性，在天然膜尼罗红。(c)2006 Elsevier B.V.保留所有权利。
The organization and dynamics of the hydrophobic fluorescent probe Nile Red incorporated in DOPC vesicles containing varying amounts of cholesterol has been monitored utilizing fluorescence-based approaches which include the red edge excitation shift (REES) approach and the parallax method for depth determination. Our results show that the fluorescence emission maximum, intensity, polarization, and lifetime of Nile Red vary with the cholesterol content of the membrane. Interestingly, Nile Red exhibits significant REES independent of the presence of cholesterol. This indicates that Nile Red is localized in a motionally restricted environment in the membrane. This is supported by analysis of membrane penetration depth of Nile Red using the parallax method which points out to a membrane interfacial localization of Nile Red. These results could be useful in analyzing membrane organization and heterogeneity in natural membranes using Nile Red. (c) 2006 Elsevier B.V. All rights reserved.