A molecular phylogenetic study of the Ephedra distachya/E. sinica complex in Eurasia
A molecular phylogenetic study of the Ephedra distachya/E. sinica complex in Eurasia
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DOI：
10.3372/wi.41.41201
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发表时间：
2011-12-01
期刊：
影响因子：
1.9
通讯作者：
Freitag, Helmut
中科院分区：
文献类型：
--
作者：
Kakiuchi, Nobuko;Mikage, Masayuki;Freitag, Helmut
本研究基于序列变异和基于形态的个体划分，为麻黄/中华麻黄复合体(麻黄科、裸子植物)的系统发育提供了新的信息。我们总共收集了来自这一复杂和密切相关物种的50个样本，其中39个样本是首次测序。与以前的研究相比，我们的抽样方案在物种数量和每个物种的样本数量方面提供了更广泛的抽样。本文首次给出了达乌里棘豆和拟稻瘟病菌的序列。我们测序了核糖体内部转录间隔区(nr ITS1+ITS2)以及RNL和TRNF之间的叶绿体基因间隔区(cp trnL-F)，但只有ITS1为系统发育重建提供了足够的信息。我们的数据首次显示：(1)一个支持良好的球果E.strobilacea/E.sarcocarpa/E.transitoria作为“L”或“Asia 2”分支的姊妹枝，此外(2)一个独立的E.disachya分支，(3)一个还包括E.sinica和部分中间E.的复合E.dahurica分支，(4)一个支持良好的E.regeliana分支和(5)一个未分解的中间E.intermedia、E.przewalskii、E.lomatolepis和E.Posodisachya的聚集体。最值得注意的是从分子数据中获得的支持，用于(1)将迄今可疑的伪地老虎与地被毛地老虎和达乌里卡地龙分离，以及(2)地被地龙和地地龙的高度序列相似性。根据形态、分子、生态和生物地理证据，中华刺参被归为达乌里刺参的一个亚种。
This study provides new information on phylogenetic relationships in the Ephedra distachya / E. sinica complex (Ephedraceae, gymnosperms) based on sequence variation and morphology-based delimitation of individual species. Altogether we have included 50 samples from this complex and closely related species with 39 samples sequenced for the first time. Our sampling scheme provides a much broader sampling both in respect of the number of species as well as the number of samples per species than has been accomplished in previous studies. Sequences for E. dahurica and E. pseudodistachya are given here for the first time. We sequenced the nuclear ribosomal internal transcribed spacer regions (nr ITS1+ITS2) as well as the chloroplast intergenic spacer between rnL and trnF (cp trnL-F), but only ITS1 was sufficiently informative for phylogeny reconstruction. Our data show, for the first time, (1) a well supported E. strobilacea / E. sarcocarpa / E. transitoria clade as sister to the "L" or "Asia 2" clade, furthermore (2) a distinct E. distachya clade, (3) a composite E. dahurica clade that also includes E. sinica and a fraction of E. intermedia, (4) a well supported E. regeliana clade and (5) an unresolved agglomeration of E. intermedia, E. przewalskii, E. lomatolepis and E. pseudodistachya. Most noteworthy is the support derived from the molecular data for (1) the separation of the hitherto doubtful E. pseudodistachya from E. distachya and E. dahurica and (2) the high degree of sequence similarity of E. sinica and E. dahurica. Based on morphological, molecular, ecological and biogeographical evidence, E. sinica is reduced to a subspecies of E. dahurica.