Bioactive properties of peptides obtained by enzymatic hydrolysis from protein byproducts of Porphyra columbina
Bioactive properties of peptides obtained by enzymatic hydrolysis from protein byproducts of Porphyra columbina
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DOI：
10.1016/j.foodres.2012.07.003
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发表时间：
2012-11-01
影响因子：
8.1
通讯作者：
Drago, Silvina R.
中科院分区：
文献类型：
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作者：
Cian, Raul E.;Martinez-Augustin, Olga;Drago, Silvina R.
从海藻中获取藻胶体的传统方法需要用冷水和热水进行连续的提取步骤。第一种冷水提取物不含藻胶体，但富含蛋白质，被认为是浪费。使用胰蛋白酶、碱性酶和这两种酶的组合，从源自哥伦比亚紫菜的第一冷水蛋白提取物(PF)中依次添加四种水解产物。 PF 水解产物 (PFH) 富含含有天冬氨酸的低分子量肽。丙氨酸和谷氨酸。 PF 和 PFH 均对大鼠脾细胞显示出免疫抑制作用，因为它们增强了 IL-10 的产生，同时抑制了 TNF α 和 IFN γ 的产生。 PFH 的这些免疫抑制作用更高。 PFH 具有抗高血压活性（>35% 的 ACE 抑制作用）和抗氧化能力（DPPH、TEAC、ORAC 和铜螯合活性）。水解可用作从藻类蛋白质副产物中获取生物活性肽的方法，并为藻胶体提取过程增加价值。 (C) 2012 Elsevier Ltd. 保留所有权利。
The traditional method to obtain phycocolloids from seaweeds implies successive extraction steps with cold and hot water. The first cold water extract has no phycocolloids but is rich in proteins and is considered a waste. Four hydrolysates were obtained using trypsin, alcalase and a combination of both sequentially added from a first cold water protein extract (PF) derived from Porphyra columbina. PF hydrolysates (PFH) were enriched in peptides with low molecular weight containing Asp. Ala and Glu. Both PF and PFH showed immunosuppressive effects on rat splenocytes as they enhanced IL-10 production while the production of TNF alpha and IFN gamma was inhibited. These immunosuppressive effects were higher for PFH. PFH had antihypertensive activity (>35% of ACE inhibition) and antioxidant capacity (DPPH, TEAC, ORAC and copper-chelating activity). The hydrolysis could be used as a mean to obtain bioactive peptides from algae protein byproducts and to add value to the phycocolloids extraction process. (C) 2012 Elsevier Ltd. All rights reserved.