Identification of proteins according to biological activity following separation by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis: analysis of human exocrine pancreatic proteins.
Identification of proteins according to biological activity following separation by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis: analysis of human exocrine pancreatic proteins.
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通过二维等电聚焦/十二烷基硫酸钠凝胶电泳分离后根据生物活性鉴定蛋白质：人外分泌胰腺蛋白的分析。
DOI：
10.1016/0003-2697(81)90298-0
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发表时间：
1981
影响因子：
2.9
通讯作者：
Bieger,W
中科院分区：
文献类型：
--
作者：
Scheele,G;Pash,J;Bieger,W
描述了一种新的方法，其中蛋白质可以根据其生物活性进行鉴定后，在两个维度上使用等电聚焦和聚丙烯酰胺凝胶电泳在十二烷基硫酸钠（G. Scheele，1975，生物化学杂志，250，5375-5385）。该程序包括用于二维凝胶斑点可视化的最佳染色方法，该方法避免使用化学固定剂，以及用于蛋白质洗脱和复性的一步法。从人胰液中分离的20种蛋白质中，有15种蛋白质被成功地鉴定出来。
A novel procedure is described whereby proteins can be identified according to their biological activity after their separation in two dimensions using isoelectric focusing and polyacrylamide gel electrophoresis in sodium dodecyl sulfate (G. Scheele, 1975, J. Biol. Chem., 250, 5375–5385). This procedure includes an optimal staining method for the visualization of two-dimensional gel spots, which avoids the use of chemical fixatives, and a one-step method for elution and renaturation of proteins. Fifteen out of the twenty discrete proteins separated from human pancreatic juice by the two-dimensional gel method were successfully identified by this procedure.