Recent developments in the transparent-chamber technique as adapted to the mouse.
Recent developments in the transparent-chamber technique as adapted to the mouse.
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适应小鼠的透明室技术的最新进展。
DOI：
10.1093/jnci/10.2.225
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发表时间：
1949
期刊：
影响因子：
--
通讯作者：
F. Legallais
中科院分区：
文献类型：
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作者：
G. H. Algire;F. Legallais
继将该技术应用于小鼠之后，又将其应用于与正常组织和肿瘤组织的生长、血管发育和循环生理有关的各种问题（，1 -5）。这在体内的肿瘤诱导和生长的问题显微镜的方法是针对一个动态的，功能性的研究在活组织中的变化。在这些研究中，使用Chalkley（6）描述的方法，通过测量功能性血管百分比的变化来补充活组织的形态学观察。测量正常组织和肿瘤组织的动脉、静脉和毛细血管的外周血压，以及记录小动脉血管活动的频率和持续时间的方法已经发展起来，在其应用过程中，这项技术已经有了许多改进。这些改进涉及增加室的有用时间，修改结构细节，缩短手术程序，通过改进显微镜和通过设计其中活组织薄层可供观察的室来提高细胞细节的分辨率，以及改进摄影设备的设计和用于静止和运动显微摄影的程序。本文根据7年来的持续使用情况，详细描述了目前使用的程序。
Adaptation to the mouse of the transparent-chamber technique (1) has been followed by its application to a variety of problems concerned with the growth, vascular development, and circulatory physiology of normal and neoplastic tissues (, l-5). This in vivo microscopic approach to problems of tumor induction and growth is directed toward a dynamic, functional study of changes in living tissues. In these studies morphologic observation of the living tissues has been supplemented by measurements of changes in the percentage of functional blood vessels, using the method described by Chalkley (6). Methods have been developed (7) for measurements of peripheral blood pressure in arteries, veins, and capillaries of normal and neoplastic tissues, and for recording frequency and duration of arteriolar vasomotor activity.Numerous improvements in the technique have been made in the course of its application. The improvements are concerned with increasing the useful duration of the chambers, modified construction details, shortened operative procedures, increased resolution of cellular detail through improvements in microscopy and through devising chambers in which thin layers of living tissue are made accessible to observation, and improved designs of photographic equipment and procedures for still and motion photomicrography. The present article is concerned with a detailed description of the procedure as now used, based on continued use over a period of 7 years.