Butyltin exposure causes a rapid decrease in cyclic AMP levels in human lymphocytes.
Butyltin exposure causes a rapid decrease in cyclic AMP levels in human lymphocytes.
复制标题
DOI：
10.1006/taap.2000.9121
复制
发表时间：
2001-03
影响因子：
3.8
通讯作者：
M. Whalen;B. Loganathan
中科院分区：
文献类型：
--
作者：
M. Whalen;B. Loganathan
自然杀伤细胞（NK）是淋巴细胞的一个子集，能够杀死肿瘤细胞、病毒感染细胞和抗体包被细胞。丁胺素（bt）用于各种消费品和工业应用。三丁基锡（TBT）存在于乳制品、肉类和鱼类中。二丁基锡（DBT）存在于塑料制品、生产过程中储存在PVC管道中的饮料和家禽产品中。bt似乎增加了接触者患癌症和病毒感染的风险。这种增加的风险可能部分是由于这些化合物对NK细胞的细胞毒性功能的抑制作用。NK细胞暴露于200 nM TBT或1.5微米DBT 24小时后，NK细胞的细胞毒功能下降了90%以上。高浓度的TBT和DBT在暴露1小时后降低了NK细胞的细胞毒功能（超过90%）。暴露于TBT或DBT 24小时可使细胞内ATP水平降低约30%。然而，暴露于300 nM TBT或10微米DBT长达1小时均未引起ATP水平的显著降低。因此，ATP水平的降低是BT暴露的长期后果。在暴露于TBT或DBT的5分钟内，细胞内cAMP水平下降多达80%。NK细胞中cAMP水平的快速下降可能是BT暴露的结果，这与NK细胞细胞毒性功能的快速下降有关。
Natural killer (NK) cells are a subset of lymphocytes that are capable of killing tumor cells, virally infected cells, and antibody-coated cells. Butyltins (BTs) are used in a variety of consumer products and industrial applications. Tributyltin (TBT) is found in dairy products, meat, and fish. Dibutyltin (DBT) is found in plastic products, beverages stored in PVC pipes during manufacturing, and poultry products. BTs appear to increase the risk of cancer and viral infections in exposed individuals. This increased risk may be due in part to the inhibitory effect of these compounds on the cytotoxic function of NK cells. A 24-h exposure of NK cells to 200 nM TBT or 1.5 microM DBT decreased the cytotoxic function of NK cells by greater than 90%. Higher concentrations of TBT and DBT decreased the cytotoxic function of NK cells (by greater than 90%) after only a 1-h exposure. A 24-h exposure to either TBT or DBT decreased intracellular ATP levels by about 30%. However, as much as a 1-h exposure to either 300 nM TBT or 10 microM DBT caused no significant decrease in ATP levels. Thus, a decrease in ATP levels is a longer-term consequence of BT exposure. Intracellular levels of cAMP are decreased by as much as 80% within 5 min of exposure to either TBT or DBT. This rapid decline in cAMP levels in NK cells may be a consequence of BT exposure that is related to the rapid decrease in the cytotoxic function of NK cells.