Mutations in a C-elegans G(q)alpha gene disrupt movement, egg laying, and viability
Mutations in a C-elegans G(q)alpha gene disrupt movement, egg laying, and viability
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DOI：
10.1016/s0896-6273(00)80123-3
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发表时间：
1996-05-01
期刊：
影响因子：
16.2
通讯作者：
Simon, MI
中科院分区：
文献类型：
--
作者：
Brundage, L;Avery, L;Simon, MI
我们发现线虫EGL-30编码的异源三聚体G蛋白α亚基与哺乳动物G(Q)α家族蛋白有80%以上的同源性，并且在COS-7细胞中可以作为G(Q)α亚基发挥作用。我们已经在线虫乙酰胆碱反应相关基因的选择中发现了新的EGL-30等位基因。两个EGL-30等位基因导致G(Q)α提前终止，在纯合子中基本上是致命的。其他6个EGL-30等位基因纯合的动物是活的和有生育能力的，但表现出产卵延迟和留下平坦的足迹。过度表达野生型EGL-30基因会产生相反的行为。对这些突变体的分析表明，它们的表型反映了肌肉或神经肌肉连接的缺陷。
We find that C. elegans egl-30 encodes a heterotrimeric G protein alpha subunit more than 80% identical to mammalian G(q) alpha family proteins, and which can function as a G(q) alpha subunit in COS-7 cells. We have identified new egl-30 alleles in a selection for genes involved in the C. elegans acetylcholine response. Two egl-30 alleles specify premature termination of G(q) alpha and are essentially lethal in homozygotes. Animals homozygous for six other egl-30 alleles are viable and fertile, but exhibit delayed egg laying and leave flattened tracks. Overexpression of the wild-type egl-30 gene produces the opposite behavior. Analysis of these mutants suggest that their phenotypes reflect defects in the muscle or neuromuscular junction.