Evidence for multiple functional copies of the mole sex-determining locus, Sry, in African murine rodents
Evidence for multiple functional copies of the mole sex-determining locus, Sry, in African murine rodents
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DOI：
10.1007/pl00006202
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发表时间：
1997-07-01
影响因子：
3.9
通讯作者：
Tucker, PK
中科院分区：
文献类型：
--
作者：
Lundrigan, BL;Tucker, PK
Southern杂交数据表明，雄性性别决定基因Sry在啮齿类动物中经常重复。在这里，我们探索了从六种非洲小鼠分离的Sry的同源和同源拷贝的DNA序列进化。聚合酶链式反应扩增和直接测序显示每个物种有两到四个Sry拷贝。所有拷贝都包括一个长的开放阅读框，其终止密码子与家鼠Mus Musculus的终止密码子非常吻合，Mus Musculus是一种已知只有一个sr？拷贝的物种：系统发育分析表明，在这类啮齿动物中至少有7个与sry相似的拷贝。假设的直系同源基因是相同的；假设的仿射基因之间的序列差异在1%到8%之间(不包括CAG重复)，高迁移率组(HMG-box)区域的差异水平比C-末端区域的低得多。这两个区域的核苷酸替换比例很高，导致氨基酸替换。长长的开放阅读框架，保守的HMG-box，以及假定的拟声词的进化模式表明它们是有功能的。
Southern hybridization data suggest that the male sex-determining locus, Sry, is often duplicated in rodents. Here we explore DNA sequence evolution of orthologous and paralogous copies of Sry isolated from six species of African murines. PCR amplification followed by direct sequencing revealed from two to four copies of Sry per species. All copies include a long open reading frame, with a stop codon that coincides closely with the stop codon of the house mouse, Mus musculus, a species known to have a single copy of Sr?: A phylogenetic analysis suggests that there are at least seven paralogous copies of Sry in this group of rodents. Putative orthologues are identical; sequence divergence among putative paralogues ranges from 1 to 8% (excluding the CAG repeat), with much lower levels of divergence in the high-mobility group (HMG-box) region than in the C-terminal region. A high proportion of nucleotide substitutions in both regions result in aminoacid replacement. The long open reading frame, conserved HMG-box, and pattern of evolution of the putative paralogues suggest that they are functional.