Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait
Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait
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苦参碱印迹整体固定相用于从苦参中提取和纯化苦参碱
DOI：
10.1016/j.jchromb.2011.02.041
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发表时间：
2011-04-15
影响因子：
3
通讯作者：
Chang, Chun
中科院分区：
文献类型：
--
作者：
Fu, Qiang;Fang, Qi;Chang, Chun
以苦参碱为模板分子，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂，甲苯和十二醇为致孔溶剂，采用原位聚合法制备了苦参碱印迹整体固定相（MIP），用于苦参碱的提取和纯化。扫描电镜研究表明，该材料具有介孔和直径36 m的结节的整体结构。采用高效液相色谱法（HPLC）研究了分子识别过程和不同色谱条件对分离的影响。MIP整体腔中的氢键、静电、疏水相互作用和分子形状匹配被认为是识别机制的原因。研究了MIP整体柱固相萃取（SPE）吸附剂对苦参碱的萃取纯化。提取率为89.2% （3.0 mmol（-1）苦参碱），富集系数为29。(C) 2011 Elsevier B.V.版权所有
A matrine-imprinted monolithic stationary phase (MIP monolith) was prepared by in situ polymerization for extraction and purification of matrine from Sophorae flavescentis Aft. Matrine was used as the template molecule, methacrylic acid as the function monomer, ethylene glycol dimethacrylate as the cross-linking agent, and toluene and dodecanol as the porogenic solvents. Scanning electron microscope study revealed that a monolithic structure with mesopores and 36 m diameter nodules was obtained. The molecular recognition process and the effect of varying chromatographic conditions on separation were examined by high-performance liquid chromatography (HPLC). Hydrogen bonding, electrostatic, hydrophobic interactions and the molecular shape matching in MIP monolith cavities were proposed to be responsible for the recognition mechanism. The use of MIP monolith as a solid-phase extraction (SPE) sorbent for extraction and purification of matrine from S.flavescentis Ait was investigated. The extraction yield was 89.2% (for 3.0 mmoll(-1) matrine) with enrichment factor 29. (C) 2011 Elsevier B.V. All rights reserved.