Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus.
Transcriptomes and DNA methylomes in apomictic cells delineate nucellar embryogenesis initiation in citrus.
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柑橘无融合生殖细胞转录组和DNA甲基化组描述珠心胚发生的起始。
DOI：
10.1093/dnares/dsab014
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发表时间：
2021-09-13
期刊：
影响因子：
--
通讯作者：
Guo WW
中科院分区：
文献类型：
--
作者：
Jia HH;Xu YT;Yin ZP;Wu XM;Qing M;Fan YJ;Song X;Xie KD;Xie ZZ;Xu Q;Deng XX;Guo WW
摘要柑橘珠心多胚性（NPE）是柑橘体细胞珠心细胞通过不定胚发生在种子中形成无性胚的一种孢子体无融合生殖方式。NPE允许砧木的克隆繁殖，但它阻碍了柑橘杂交育种。为了了解参与NPE启动的细胞过程，我们分析了激光显微切割捕获的柑橘无融合生殖细胞中的转录组和DNA甲基化组。在无融合生殖细胞中，核糖体生物合成和蛋白质降解被激活，而生长素极性运输被抑制。活性氧（ROS）在多胚胚珠中积累，引起对氧化胁迫的反应。整体DNA甲基化水平，尤其是CHH背景的甲基化水平下降，而NPE控制关键基因CitRWP的甲基化水平上升。在无融合生殖细胞中特异性共表达的含C2H2结构域的转录因子基因和CitRWP可以协调启动NPE。胚性发育和胼胝质沉积过程的激活表明了珠心胚起始细胞的胚性命运。在我们的柑橘NPE起始的工作模型中，DNA超甲基化可以激活CitRWP的转录，其增加C2H2表达和ROS积累，触发表观遗传调控并调节无融合生殖细胞中的细胞命运转变和NEI细胞身份。
Abstract Citrus nucellar poly-embryony (NPE) is a mode of sporophytic apomixis that asexual embryos formed in the seed through adventitious embryogenesis from the somatic nucellar cells. NPE allows clonal propagation of rootstocks, but it impedes citrus cross breeding. To understand the cellular processes involved in NPE initiation, we profiled the transcriptomes and DNA methylomes in laser microdissection captured citrus apomictic cells. In apomictic cells, ribosome biogenesis and protein degradation were activated, whereas auxin polar transport was repressed. Reactive oxygen species (ROS) accumulated in the poly-embryonic ovules, and response to oxidative stress was provoked. The global DNA methylation level, especially that of CHH context, was decreased, whereas the methylation level of the NPE-controlling key gene CitRWP was increased. A C2H2 domain-containing transcription factor gene and CitRWP co-expressed specifically in apomictic cells may coordinate to initiate NPE. The activated embryogenic development and callose deposition processes indicated embryogenic fate of nucellar embryo initial (NEI) cells. In our working model for citrus NPE initiation, DNA hyper-methylation may activate transcription of CitRWP, which increases C2H2 expression and ROS accumulation, triggers epigenetic regulation and regulates cell fate transition and NEI cell identity in the apomictic cells.
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影响因子：
12.3
作者：
Niederhuth CE;Bewick AJ;Ji L;Alabady MS;Kim KD;Li Q;Rohr NA;Rambani A;Burke JM;Udall JA;Egesi C;Schmutz J;Grimwood J;Jackson SA;Springer NM;Schmitz RJ
通讯作者：
Schmitz RJ
影响因子：
2.4
作者：
Kepiro, J. L.;Roose, M. L.
通讯作者：
Roose, M. L.
影响因子：
7.2
作者：
Koszegi, David;Johnston, Amal J.;Baeumlein, Helmut
通讯作者：
Baeumlein, Helmut
影响因子：
2.4
作者：
Kumar, Vajinder;Malik, Surendra Kumar;Bhat, Shripad Ramachandra
通讯作者：
Bhat, Shripad Ramachandra
影响因子：
7.4
作者：
Mau, Martin;Corral, Jose M.;Sharbel, Timothy F.
通讯作者：
Sharbel, Timothy F.