Mouse Models for Atopic Dermatitis.
Mouse Models for Atopic Dermatitis.
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特应性皮炎小鼠模型。
DOI：
10.1002/cpz1.709
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Nagao,Keisuke
中科院分区：
文献类型：
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作者：
Sakamoto,Keiko;Nagao,Keisuke
特应性皮炎（AD）是一种多因素疾病，具有潜在的屏障破坏和微生物菌群改变，导致皮肤干燥和湿疹性炎症并持续瘙痒。小鼠模型已被大量用于研究阿尔茨海默病的病理生理。在各种AD小鼠模型中，外用钙三醇（一种维生素D3类似物，在实验环境中称为MC903）诱导的AD样炎症是一种通用模型，可应用于任何品系的小鼠，可用于免疫学和形态学研究。在此，我们提供了MC903局部应用的基本方案和评估表型的方法。诱导AD样炎症后，采集皮肤进行流式细胞术分析，以及组织学和免疫荧光显微镜分析。这些方法的结合能够准确表征炎症程度、炎症浸润类型和免疫浸润的定位。2023年出版。这篇文章是美国政府的作品，在美国属于公有领域。基本方案1：应用MC903和大体表型评估基本方案2：处理皮肤进行流式细胞术分析支持方案：皮肤免疫细胞表面染色和流式细胞术分析基本方案3：收获皮肤进行组织学分析基本方案4：免疫荧光染色以识别免疫细胞浸润
Atopic dermatitis (AD) is a multifactorial disease with underlying barrier disruption and altered microbial flora, resulting in dry skin and eczematous inflammation with persistent pruritis. Mouse models have been heavily used to investigate AD pathophysiology. Among various AD mouse models, AD‐like inflammation induced by topical calcipotriol, a vitamin D3 analog referred to as MC903 in experimental settings, is a versatile model that can be applied to any strain of mice, which can be used for immunologic and morphologic studies. Herein, we provide basic protocols for the topical application of MC903 and approaches to assess phenotypes. After inducing AD‐like inflammation, the skin is harvested for flow cytometry analysis, as well as for histologic and immunofluorescence microscopy analyses. The combination of these approaches enables accurate characterization of the degree of inflammation, type of inflammatory infiltrate, and localization of immune infiltrates. Published 2023. This article is a U.S. Government work and is in the public domain in the USA.Basic Protocol 1: Application of MC903 and gross phenotype assessmentBasic Protocol 2: Processing skin for flow cytometry analysisSupport Protocol: Skin immune cell surface staining and flow cytometry analysisBasic Protocol 3: Harvesting skin for histologic analysisBasic Protocol 4: Immunofluorescence staining to identify immune cell infiltrates