Addition of triglycerides with arachidonic acid or docosahexaenoic acid to infant formula has tissue- and lipid class-specific effects on fatty acids and hepatic desaturase activities in formula-fed piglets
Addition of triglycerides with arachidonic acid or docosahexaenoic acid to infant formula has tissue- and lipid class-specific effects on fatty acids and hepatic desaturase activities in formula-fed piglets
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DOI：
10.1093/jn/128.8.1376
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发表时间：
1998-08-01
影响因子：
4.2
通讯作者：
Rioux, FM
中科院分区：
文献类型：
--
作者：
de la Presa-Owens, S;Innis, SM;Rioux, FM
研究配方中添加甘油三酯与花生四烯酸[20：4(n-6)]或二十二碳六烯酸[22：6(n-3)]对乳糜粒、低密度脂蛋白、高密度脂蛋白、肝、心、肾、脑(n-6)和(n-3)脂肪酸的影响。仔猪从出生到18日龄，分别饲喂20%18：2(n-6)和2%18：3(n-3)配方，不添加或不添加0.8%20：4(n-6)或0.3%22：6(n-3)。在配方中添加20：4(n-6)或22：6(n-3)的效果因组织和脂肪的不同而不同，大脑表现出对变化的抵抗力，20：4(n-6)配方饲喂的仔猪血浆、心脏和肾脏磷脂和甘油三酯显著高于对照组。肝脏甘油三酯20：4(n-6)，但血浆和组织磷脂18：2(n-6)低于不饲喂20：4(n-6)配方的仔猪。相反，补充22：6(n-3)对血浆或组织18：2(n-6)没有影响。与不饲喂22：6(n-3)相比，饲喂22：6(n-3)配方的仔猪肝脏磷脂(高30-92%)和甘油三酯(高200%)的含量较高，同时肝脏磷脂酰乙醇胺(平均+/-扫描电子显微镜，分别为8.6+/-0.4和10.5+/-0.4%)的含量较低，但甘油三酯(5.2+/-0.4和11.5+/-0.5%)的含量较高。较高的肝、心、肾磷脂含量为20：5(n-3)。这些结果表明，饲料20：4(n-6)和组织18：2(n-6)之间以及饲料20：4(n-6)和组织20：5(n-3)之间存在竞争性相互作用，而不是22：6(n-3)。结果还表明，即使在低摄入量的情况下，补充22：6(n-3)或20：4(n-6)也会改变组织磷脂20：4(n-6)至20：5(n-3)的平衡。研究日粮中添加20：4(n-6)和22：6(n-3)的生理效应时，应考虑不同组织对饲料脂肪酸的不同敏感性。
The effects of including triglycerides with arachidonic [20:4(n-6)] or docosahexaenoic acid [22:6(n-3)] in formula on plasma chylomicron, LDL and HDL, liver, heart, kidney and brain (n-6) and (n-3) fatty acids were investigated in formula-fed piglets. Piglets were fed formula with (in % total fatty acids) 20% 18:2(n-6) and 2% 18:3(n-3) without or with 0.8% 20:4(n-6) or 0.3% 22:6(n-3) from birth to 18 d. The effects of adding 20:4(n-6) or 22:6(n-3) to the formula differed among different tissues and lipids, with the brain showing resistance to change, Piglets fed formula with 20:4(n-6) had significantly higher plasma, heart and kidney phospholipid and triglyceride, and liver triglyceride 20:4(n-6), but lower plasma and tissue phospholipid 18:2(n-6) than piglets fed formula without 20:4(n-6). Supplementation with 22:6(n-3), in contrast, had no effect on plasma or tissue 18:2(n-6). Higher 22:6(n-3) in liver phospholipid (30-92% greater) and triglyceride (200% greater) in piglets fed formula with 22:6(n-3) rather than without 22:6(n-3) was accompanied by lower 20:4(n-6) in liver phosphatidylethanolamine (mean +/- SEM, 8.6 +/- 0.4 and 10.5 +/- 0.4% fatty acids, respectively), but higher 20:4(n-6) in triglyceride (5.2 +/- 0.4 and 11.5 +/- 0.5%, respectively), and higher liver, heart and kidney phospholipid 20:5(n-3). These results indicate competitive interaction between dietary 20:4(n-6) and tissue 18:2(n-6), and between dietary 20:4(n-6) and tissue 20:5(n-3), rather than 22:6(n-3). The results also show that even at low intakes, dietary 22:6(n-3) or 20:4(n-6) supplementation alters the tissue phospholipid 20:4(n-6) to 20:5(n-3) balance. Studies on the physiologic effects of dietary 20:4(n-6) and 22:6(n-3) supplementation should consider the different sensitivity among tissues to dietary fatty acids.