Identification of TgCBAP, a novel cytoskeletal protein that localizes to three distinct subcompartments of the Toxoplasma gondii pellicle.
Identification of TgCBAP, a novel cytoskeletal protein that localizes to three distinct subcompartments of the Toxoplasma gondii pellicle.
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DOI：
10.1371/journal.pone.0098492
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ward GE
中科院分区：
文献类型：
--
作者：
Tilley LD;Krishnamurthy S;Westwood NJ;Ward GE
弓形虫和相关的顶端复合体寄生虫的细胞骨架是高度极化的，顶端和基底部由不同的蛋白质复合体组成。已知这些复合体的成分在寄生虫的形状、细胞分裂和宿主细胞入侵中发挥重要作用。在寻找弓形虫侵袭小分子抑制物(Conoidin A)的生物相关靶点的过程中，我们发现了一种新的细胞骨架蛋白，我们将其命名为TgCBAP(保守性碱基尖端外周蛋白)。TgCBAP的同源基因只存在于其他顶端复合体的基因组中；它们不包含可识别的结构域或基序，其功能尚不清楚(S)。作为阐明这个高度保守的蛋白质家族功能的第一步，我们通过双同源重组破坏了TgCBAP基因。缺乏TgCBAP的寄生虫对Conoidin A的作用与野生型一样敏感，这表明TgCBAP不是Conoidin A的生物学相关靶标。然而，ΔTgCBAP寄生虫比野生型寄生虫短得多，而且在培养过程中存在生长缺陷。此外，TgCBAP具有不同寻常的亚细胞定位，在寄生虫的顶端和底端形成小环，并定位于寄生虫外围的点状环状结构。这些数据确定了弓形虫顶端和基本亚室的新标记，揭示了沿寄生虫外围的一个潜在的新亚室，并确认TgCBAP是最有效的裂解周期所需的寄生虫大小的决定因素。
The cytoskeletons of Toxoplasma gondii and related apicomplexan parasites are highly polarized, with apical and basal regions comprised of distinct protein complexes. Components of these complexes are known to play important roles in parasite shape, cell division, and host cell invasion. During an effort to discover the biologically relevant target of a small-molecule inhibitor of T. gondii invasion (Conoidin A), we discovered a novel cytoskeletal protein that we named TgCBAP (Conserved Basal Apical Peripheral protein). Orthologs of TgCBAP are only found in the genomes of other apicomplexans; they contain no identifiable domains or motifs and their function(s) is unknown. As a first step toward elucidating the function of this highly conserved family of proteins, we disrupted the TgCBAP gene by double homologous recombination. Parasites lacking TgCBAP are as sensitive to the effects of Conoidin A as wild-type parasites, demonstrating that TgCBAP is not the biologically relevant target of Conoidin A. However, ΔTgCBAP parasites are significantly shorter than wild-type parasites and have a growth defect in culture. Furthermore, TgCBAP has an unusual subcellular localization, forming small rings at the apical and basal ends of the parasite and localizing to punctate, ring-like structures around the parasite periphery. These data identify a new marker of the apical and basal subcompartments of T. gondii, reveal a potentially novel compartment along the parasite periphery, and identify TgCBAP as a determinant of parasite size that is required for a maximally efficient lytic cycle.
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弓形虫 IMC 蛋白的一个新家族在协调寄生虫分裂中表现出层次结构和功能。
影响因子：
6.7
作者：
Beck, Josh R.;Rodriguez-Fernandez, Imilce A.;de Leon, Jessica Cruz;Huynh, My-Hang;Carruthers, Vern B.;Morrissette, Naomi S.;Bradley, Peter J.
通讯作者：
Bradley, Peter J.
影响因子：
1.5
作者：
Gubbels, MJ;Wieffer, M;Striepen, B
通讯作者：
Striepen, B
DOI：
10.1007/bf00380795
发表时间：
1977-01-01
期刊：
ZEITSCHRIFT FUR PARASITENKUNDE-PARASITOLOGY RESEARCH
影响因子：
--
作者：
PORCHET, E;TORPIER, G
通讯作者：
TORPIER, G
影响因子：
--
作者：
Huynh, My-Hang;Carruthers, Vern B.
通讯作者：
Carruthers, Vern B.
影响因子：
--
作者：
Fox, Barbara A.;Ristuccia, Jessica G.;Bzik, David J.
通讯作者：
Bzik, David J.