MOLECULAR ANALYSIS OF THE ALCOHOL-DEHYDROGENASE (ADHL) GENE OF MAIZE
MOLECULAR ANALYSIS OF THE ALCOHOL-DEHYDROGENASE (ADHL) GENE OF MAIZE
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DOI：
10.1093/nar/12.9.3983
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
PEACOCK, WJ
中科院分区：
文献类型：
--
作者：
DENNIS, ES;GERLACH, WL;PEACOCK, WJ
构建了包含该基因全长蛋白编码区的maizeAdhl基因的cDNA克隆。从核苷酸序列预测的蛋白质序列与有限的ADH1酶的蛋白质测序数据一致。以该克隆为探针，获得了含Adhl基因的11.5kb基因组片段，并对该基因进行了全序列测定。该基因被9个内含子打断，它们的连接序列符合动物基因的共识序列。在该基因内有一个跨越内含子-外显子连接的片段(104bp)的三倍体。推测的启动子元件已经被确定，并且在核苷酸序列和位置上与其他植物和动物基因的核苷酸序列和位置相似，相对于转录的开始。没有明显可识别的聚(A)添加信号。对来自Adhl-Falle的cDNA和来自AnAdhl-Salle的基因组克隆的核苷酸序列进行比较，发现了氨基酸差异，这与观察到的两种酶的电泳迁移率的差异一致。玉米ADH1的氨基酸序列与马肝ADH有50%的同源性，而与酵母ADH的同源性只有20%。
A cDNA clone of maizeAdhlwhich contains the entire protein coding region of the gene has been constructed. The protein sequence predicted from the nucieotide sequence is in agreement with limited protein sequencing data for the ADH1 enzyme. An 11.5 kb genomic fragment containing theAdhlgene has been isolated using the cDNA clone as a probe, and the gene region fully sequenced. The gene is interrupted by 9 introns, their junction sequences fitting the animal gene consensus sequence. Within the gene there 1s a triplication of a segment (104 bp) spanning an intron-exon junction. Presumptive promoter elements have been identified and are similar in nucleotide sequence and location, relative to the start of transcription, to those of other plant and animal genes. No recognizable poly(A ) addition signal is evident. Comparison of the nucleotide sequences of the cDNA (derived from anAdhl-Fallele) and genomic (derived from anAdhl-Sallele) clones has identified an amino acid difference consistent with the observed difference in electrophoretic mobility of the two enzymes. The maize ADH1 amino acid sequence is 50% homologous to that of horse liver ADH but is only 20% homologous to yeast ADH.