Demonstration of elevated type I and type III procollagen mRNA levels in cutaneous wounds treated with helium-neon laser. Proposed mechanism for enhanced wound healing.
Demonstration of elevated type I and type III procollagen mRNA levels in cutaneous wounds treated with helium-neon laser. Proposed mechanism for enhanced wound healing.
复制标题
证明用氦氖激光治疗的皮肤伤口中 I 型和 III 型原胶原 mRNA 水平升高。
DOI：
10.1016/s0006-291x(86)80399-0
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Uitto,J
中科院分区：
文献类型：
--
作者：
Saperia,D;Glassberg,E;Lyons,RF;Abergel,RP;Baneux,P;Castel,JC;Dwyer,RM;Uitto,J
为了评估伤口愈合的激光调制，在猪的背部产生全层皮肤伤口，并用氦氖激光进行治疗。为了进行比较，一些伤口用非激光能量源（钨丝灯）治疗或不治疗作为对照。通过与cDNA探针的分子杂交测定伤口中的I型和III型前胶原mRNA水平。结果表明，He-Ne激光治疗后17天和28天，Ⅰ型和Ⅲ型mRNA水平明显高于对照组和钨光治疗组。结果表明，氦氖激光通过增强前胶原基因表达促进伤口愈合。这些观察结果可能与先前的临床研究相关，表明氦氖激光刺激伤口愈合。
To assess laser modulation of wound healing, full-thickness cutaneous wounds were produced in the backs of pigs, and subjected to treatment with helium-neon laser. For comparison, some wounds were treated with non-laser energy source (a tungsten light) or left untreated as controls. Type I and type III procollagen mRNA levels were determined in the wounds by molecular hybridization with cDNA probes. The results indicated that type I and type III mRNA levels were markedly increased at days 17 and 28 of the healing in wounds treated with He-Ne laser, when compared to control or tungsten light-treated wounds. The results suggest that helium-neon laser stimulates wound healing by enhancing procollagen gene expression. These observations may have relevance to previous clinical studies suggesting that helium-neon laser stimulates wound healing.