Balance of two secretion pathways of nerve growth factor in PC12 cells changes during the progression of their differentiation, with a decrease in constitutive secretion in more differentiated cells
Balance of two secretion pathways of nerve growth factor in PC12 cells changes during the progression of their differentiation, with a decrease in constitutive secretion in more differentiated cells
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PC12细胞中神经生长因子的两条分泌途径的平衡在其分化过程中发生变化，分化程度较高的细胞的组成型分泌减少
DOI：
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发表时间：
2000
影响因子：
4.2
通讯作者：
S. Furukawa
中科院分区：
文献类型：
--
作者：
H. Nomoto;Kimihiko Tomotoshi;H. Ito;S. Furukawa
蛋白质通过组成型或调节性途径从动物细胞中分泌。当神经生长因子（NGF）的cDNA被引入PC12细胞时，这些细胞产生并分泌活性NGF，根据脉冲追踪和ELISA研究的结果，NGF不仅以组成型方式分泌，而且以活性依赖性调节方式分泌。调节的分泌是由去极化、环AMP类似物或β-肾上腺素能激动剂引起的，而不是由谷氨酸或卡巴胆碱引起的。由于这些转染细胞分化成的形态与用 NGF 蛋白孵育的细胞没有区别，因此我们接下来比较了 PC12 细胞在分化不同阶段的 NGF 分泌途径。 NGF-cDNA转染后第2天和第7天，NGF以组成型和调节型方式分泌，但第7天分化程度较高的细胞的NGF组成型分泌减少，成熟的NGF倾向于在细胞内积累。这些结果表明神经营养素分泌机制在PC12细胞的分化过程中受到密切调节。蛋白质分泌途径的这种变化可能对神经元的发育产生深远的作用。 J.神经科学。资源。 59:632–642, 2000 © 2000 Wiley-Liss, Inc.
Proteins are secreted from animal cells by either a constitutive or a regulated pathway. When cDNA of nerve growth factor (NGF) was introduced into PC12 cells, these cells produced and secreted active NGF, where NGF was secreted not only in constitutive but also in activity‐dependent regulated way according to the results of pulse‐chase and ELISA studies. The regulated secretion was caused by depolarization, cyclic AMP analogue, or beta‐adrenergic agonist but not by glutamate or carbachol. Because these transfected cells differentiated into a morphology indistinguishable from that incubated with NGF protein, we next compared the secretion pathways of NGF from PC12 cells at different stages of the differentiation. NGF was secreted in both constitutive and regulated way at 2 and 7 days after the transfection of NGF‐cDNA, but the constitutive secretion of NGF from the more differentiated cells of Day 7 was decreased and mature NGF tended to accumulate in the cells. These results indicate that the neurotrophin secretion mechanism is intimately regulated in the course of the differentiation of PC12 cells. Such a change in the protein secretion pathway might have an profound role in the development of neurons. J. Neurosci. Res. 59:632–642, 2000 © 2000 Wiley‐Liss, Inc.
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将天然神经生长因子前体加工成具有生物活性的神经生长因子。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Edwards,RH;Selby,MJ;Garcia,PD;Rutter,WJ
通讯作者：
Rutter,WJ
125I-神经生长因子与 PC12 嗜铬细胞瘤细胞的关联。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bernd,P;Greene,LA
通讯作者：
Greene,LA
影响因子：
11.4
作者：
SUTER, U;HEYMACH, JV;SHOOTER, EM
通讯作者：
SHOOTER, EM
逆转录病毒载体介导的 PC12 细胞的自分泌分化。
影响因子：
2.9
作者：
Short,MP;Rosenberg,MB;Ezzedine,ZD;Gage,FH;Friedmann,T;Breakefield,XO
通讯作者：
Breakefield,XO
DOI：
10.1073/pnas.95.16.9614
发表时间：
1998-08-04
影响因子：
11.1
作者：
Krüttgen, A;Möller, JC;Shooter, EM
通讯作者：
Shooter, EM