Control of cell detachment in a microfluidic device using a thermo-responsive copolymer on a gold substrate
Control of cell detachment in a microfluidic device using a thermo-responsive copolymer on a gold substrate
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DOI：
10.1039/b708619a
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Duschl, Claus
中科院分区：
文献类型：
--
作者：
Ernst, Oliver;Lieske, Antje;Duschl, Claus
细胞粘附的控制在细胞生物技术的许多过程中是至关重要的。合成了一种温度响应性的聚（N-异丙基丙烯酰胺）-聚（乙二醇）-硫醇（PNIPAAm-PEG-thiol）共聚物，用于形成自组装单分子膜（SAM），从而控制细胞在金基底上的粘附。水在这些层上的接触角在45摄氏度的65度和25摄氏度的54度之间变化。这种行为与聚合物链从延伸和高度水合到塌陷的线圈状状态的转变一致。在37摄氏度下，培养的成纤维细胞在该表面上正常粘附和扩散，并通过将温度降低到低于低临界溶解温度（LCST）而分离。层可以重复使用而不会损失其功能。为了量化共聚物层诱导细胞脱离的能力，向细胞施加限定的剪切力。为此目的，使用微流体装置中的层流。我们的方法提供了一种策略，该策略基于建立层的功能参数和分子细节之间的相关性来优化层的性质。
The control of cell adhesion is crucial in many procedures in cellular biotechnology. A thermo-responsive poly( N-isopropylacrylamide)-poly( ethylene glycol)-thiol ( PNIPAAm-PEG-thiol) copolymer was synthesized for the formation of self-assembled monolayers ( SAM) that allow the control of adhesion of cells on gold substrates. The contact angle of water on these layers varies between 65 degrees at a temperature of 45 degrees C and 54 degrees at 25 degrees C. This behaviour is consistent with a transition of the polymer chains from an extended and highly hydrated to a collapsed coil-like state. At 37 degrees C, cultivated fibroblasts adhere and spread normally on this surface and detach by reducing the temperature below the lower critical solution temperature ( LCST). Layers can repeatedly be used without loss of their functionality. In order to quantify the capability of the copolymer layer to induce cell detachment, defined shear forces are applied to the cells. For this purpose, the laminar flow in a microfluidic device is used. Our approach provides a strategy for the optimization of layer properties that is based on establishing a correlation between a functional parameter and molecular details of the layers.