Colcemid inhibits growth during early G1 in normal but not in tumorigenic lymphocytes.
Colcemid inhibits growth during early G1 in normal but not in tumorigenic lymphocytes.
复制标题
Colcemid 可抑制正常淋巴细胞在 G1 早期的生长，但不会抑制致瘤淋巴细胞的生长。
DOI：
10.1016/0014-4827(86)90220-x
复制
发表时间：
1986
影响因子：
3.7
通讯作者：
Rabbitts,TH
中科院分区：
文献类型：
--
作者：
Kenter,AL;Watson,JV;Azim,T;Rabbitts,TH
有丝分裂刺激的人和小鼠淋巴细胞通过一个新发现的亚期G1‘进入细胞周期(G0、G1a、G1B、S、G2+M)。这个亚阶段在G1a之前，不同于G0。在永生化和致瘤的淋巴母细胞系(LCL)中，根据细胞荧光标准，G1‘亚期缺失。此外，Colcemid抑制有丝分裂原刺激的淋巴细胞和LCLS中通过G0、G1‘和G2期的过渡。在G1早期，致瘤性LCL对Colcemid的生长抑制不敏感。这些观察表明，在G0G1‘期间发生了一系列渐进的变化，导致了对生长控制的放松管制。
Mitogenically stimulated human and mouse lymphocytes enter the cell cycle (G0, G1A, G1B, S, G2+ M) via a newly recognized subphase, G1′. This subphase precedes G1A and is distinct from G0. The G1′ subphase is absent in immortalized and tumorigenic lymphoblastoid cell lines (LCLs) by cytofluorimetric criteria. Furthermore, colcemid inhibits transition through the G0 G1′ as well as G2 phases in mitogen-stimulated lymphocytes and in LCLs. Tumorigenic LCLs are not sensitive to growth inhibition by colcemid during early G1. These observations suggest that a progressive series of changes have occurred during G0 G1′ which lead to deregulation of growth control.