The assessment of the chemotherapeutic effects of vinca alkaloids by immunofluorescence and flow cytometry.
The assessment of the chemotherapeutic effects of vinca alkaloids by immunofluorescence and flow cytometry.
复制标题
通过免疫荧光和流式细胞术评估长春花生物碱的化疗效果。
DOI：
10.1111/j.1749-6632.1986.tb42029.x
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发表时间：
1986
影响因子：
5.2
通讯作者：
Yunis,AA
中科院分区：
文献类型：
--
作者：
Gratzner,HG;Fountzilas,G;Stein,JH;Yunis,AA
采用流式细胞术和免疫荧光技术研究了长春花生物碱对人胰腺癌细胞株MIA Pa Ca-2 DNA合成速率的影响。该方法采用对溴脱氧尿嘧啶或碘脱氧尿嘧啶具有高度特异性的单克隆抗体。药物长春新碱和长春地碱似乎对S期DNA合成率没有直接影响，而DHAD，一种先前发现的影响DNA合成的化合物，通过这种技术似乎可以抑制S期特定片段的DNA合成。在S期或G2+ M期被阻断的细胞在细胞周期的这些阶段仍能以较高的速率结合BrdUrd，由此观察到长春新碱不会通过抑制DNA合成来阻断细胞。
The effects of the vinca alkaloids on the rates of DNA synthesis in the human pancreatic carcinoma line, MIA Pa Ca-2 have been studied by a new technique for measuring cell kinetics and DNA synthesis by flow cytometry and immunofluorescence. The method employs a monoclonal antibody that is highly specific for bromodeoxyuridine or iododeoxyuridine. The drugs vincristine and vindesine do not appear to have a direct effect on DNA synthesis rate across S phase, whereas DHAD, a compound that has been found previously to affect DNA synthesis, does appear by this technique to inhibit DNA synthesis at specific segments of S phase. That vincristine does not block cells in S phase by inhibiting DNA synthesis is borne out of the observation that cells blocked in S or G2+ M can still incorporate BrdUrd at a high rate in these phases of the cell cycle.