A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells
A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells
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DOI：
10.1093/nar/gkg375
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发表时间：
2003-06-01
影响因子：
14.9
通讯作者：
Lacroix, E
中科院分区：
文献类型：
--
作者：
Epinat, JC;Arnould, S;Lacroix, E
同源基因靶向是反向遗传学的最终工具，但其使用通常受到低效率的限制。在许多最近的研究中，特定于特定的DNA双链断裂（DSB）已用于诱导有效的基因靶向。工程高度特定，专用的DNA核丝酶是该技术更广泛使用的关键。在这项研究中，我们介绍了两种小说嵌合肠核酶，这些小核核酸酶来自归巢核酸内切核酸酶。第一个能够在酵母和哺乳动物细胞中诱导重组，而第二个则裂解了新型（选择的）DNA靶位点。这些结果是迈向定制核酸内切酶的第一步，目的是针对性的基因组工程。
Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have been used to induce efficient gene targeting. Engineering highly specific, dedicated DNA endonucleases is the key to a wider usage of this technology. In this study, we present two novel, chimeric meganucleases, derived from homing endonucleases. The first one is able to induce recombination in yeast and mammalian cells, whereas the second cleaves a novel (chosen) DNA target site. These results are a first step toward the generation of custom endonucleases for the purpose of targeted genome engineering.