Methods To Measure the Antioxidant Activity of Phytochemicals and Plant Extracts
Methods To Measure the Antioxidant Activity of Phytochemicals and Plant Extracts
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DOI：
10.1021/acs.jafc.8b01079
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发表时间：
2018-04-04
影响因子：
6.1
通讯作者：
Valgimigli, Luca
中科院分区：
文献类型：
--
作者：
Amorati, Riccardo;Valgimigli, Luca
测量植物源化合物的抗氧化特性需要适当的方法来解决抗氧化活性的机制并关注涉及抗氧化剂的反应动力学。基于抑制自动氧化的方法最适合断链抗氧化剂和终止增强抗氧化剂，而预防性抗氧化剂则需要不同的具体研究。对一系列化学测试方法进行了严格的审查，强调了它们的优点和局限性，并讨论了它们在研究纯分子和原始提取物方面的有用性。还讨论了反应介质对抗氧化剂性能的影响。
Measurement of antioxidant properties in plant-derived compounds requires appropriate methods that address the mechanism of antioxidant activity and focus on the kinetics of the reactions involving the antioxidants. Methods based on inhibited autoxidations are the most suited for chain-breaking antioxidants and for termination-enhancing antioxidants, while different specific studies are needed for preventive antioxidants. A selection of chemical testing methods is critically reviewed, highlighting their advantages and limitations and discussing their usefulness to investigate both pure molecules and raw extracts. The influence of the reaction medium on antioxidants' performance is also addressed.