Gene profiles during root canal treatment in experimental rat periapical lesions
Gene profiles during root canal treatment in experimental rat periapical lesions
复制标题
DOI：
10.1016/j.joen.2007.04.016
复制
发表时间：
2007-08-01
影响因子：
4.2
通讯作者：
Takashiba, Shogo
中科院分区：
文献类型：
--
作者：
Martinez, Zulema Rosalia Arias;Naruishi, Koji;Takashiba, Shogo
本研究旨在探讨根管治疗过程中根尖周病变的基因表达谱。根尖周病变通过暴露Sprague-Dawley大鼠的牙髓来实验诱导。3 wk后行根管充填术（RCF），1或4 wk后处死动物。从根尖周组织中提取总RNA并进行cDNA微阵列分析。组织学和放射学证实，牙髓暴露后4周（炎症期），病变扩大，并在RCF后4周（愈合期）稳定。在微阵列上的大约30，000个基因中，203个基因上调至超过5倍（例如，IL-1 β），864个基因下调至低于基线水平的20%（例如，caspase 8）。与炎症期相比，我们发现133个基因表达上调（例如，IL-1 α）和50个基因下调（例如，防御素α 5）。与基因表达谱相对应，通过免疫组织化学观察到IL-1 α和IL-1 β在根尖周病变中积聚。这些基因表达谱可能有助于根尖周病变愈合过程的诊断。
The purpose of this study was to profile gene expression in periapical lesions during root canal treatment (RCT). Periapical lesions were induced experimentally by exposing the pulp in Sprague-Dawley rats. After 3 wk, the animals received root canal filling (RCF) and were sacrificed 1 or 4 wk later. From the periapical tissues, total RNA was extracted and processed for cDNA-microarray analysis. The lesions were histologically and radiographically confirmed to expand 4 wk after pulp exposure (inflammation phase) and to stabilize 4 wk after RCF (healing phase). In approximately 30,000 genes on the microarray, 203 genes were up-regulated to more than 5-fold (e.g., IL-1 beta), and 864 genes were down-regulated to less than 20% of baseline level (e.g., caspase 8) in inflammation phase. Compared with inflammation phase, we found that 133 genes were up-regulated (e.g., IL-1 alpha) and 50 genes were down-regulated (e.g., defensin alpha 5) in healing phase. Corresponding to the gene expression profiles, accumulation of IL-1 alpha and IL-1 beta was observed in the periapical lesions by immunohistochemistry. These gene profiles might be useful in diagnosing the healing process of periapical lesions.