Whole-body energy metabolism and skeletal muscle biochemical characteristics.
Whole-body energy metabolism and skeletal muscle biochemical characteristics.
复制标题
全身能量代谢和骨骼肌生化特征。
DOI：
10.1016/0026-0495(94)90081-7
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Ravussin,E
中科院分区：
文献类型：
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作者：
Zurlo,F;Nemeth,PM;Choksi,RM;Sesodia,S;Ravussin,E
给定体型的低代谢率和低脂肪与碳水化合物氧化比是已知的体重增加的危险因素，但其潜在的生物学机制尚不清楚。比较14例受试者（7男7女，年龄30±6岁[mean±SD]， 79.1±17.3 kg，体脂22%±7%）的24小时能量消耗（24EE）、睡眠代谢率（SMR）、24小时呼吸商（24RQ）和前臂摄氧量与骨骼肌纤维类型比例和股外侧肌酶活性的关系。选择以下酶来代表主要的能量产生途径：乳酸脱氢酶（LDH）和糖酵解的磷酸果糖激酶（PFK）；柠檬酸合成酶（CS）和β-羟酸辅酶A脱氢酶（β-OAC）进行氧化；肌酸激酶（CK）和腺苷激酶（AK）负责高能磷酸盐代谢。前臂静息摄氧量与肌肉大小正相关（IIa:r= 0.55，P= 0.04; IIb:r= 0.51，P= 0.06），与慢速氧化纤维比例负相关（I:r= -）。77年,P =措施)。24EE和SMR调整了与PFK活性相关的无脂量、脂肪量、性别和年龄的差异（r= 0.56，P= 0.04和r= 0.69，P= 0.07）。24RQ与β-OAC活性呈负相关（r=−）。75年,P = .002)。我们的研究结果表明，肌肉生物化学的差异可以部分解释肌肉摄氧量和全身能量代谢（即代谢率和底物氧化）的个体差异。
A low metabolic rate for a given body size and low fat versus carbohydrate oxidation ratio are known risk factors for body weight gain, but the underlying biological mechanisms are poorly understood. Twenty-four-hour energy expenditure (24EE), sleeping metabolic rate (SMR), 24-hour respiratory quotient (24RQ), and forearm oxygen uptake were compared with respect to the proportion of skeletal muscle fiber types and the enzyme activities of the vastus lateralis in 14 subjects (seven men and seven women aged 30 ± 6 years [mean ± SD], 79.1 ± 17.3 kg, 22% ± 7% body fat). The following enzymes were chosen to represent the major energy-generating pathways: lactate dehydrogenase (LDH) and phosphofructokinase (PFK) for glycolysis; citrate synthase (CS) and β-hydroxyacl-coenzyme A dehydrogenase (β-OAC) for oxidation; and creatine kinase (CK) and adenylokinase (AK) for high-energy phosphate metabolism. Forearm resting oxygen uptake adjusted for muscle size correlated positively with the proportion of fast-twitch muscle fibers (IIa:r= .55,P= .04; IIb:r= .51,P= .06) and inversely with the proportion of slow oxidative fibers (I:r= −.77,P= .001). 24EE and SMR adjusted for differences in fat-free mass, fat mass, sex, and age correlated with PFK activity (r= .56,P= .04 andr= .69,P= .007, respectively). 24RQ correlated negatively with β-OAC activity (r= −.75,P= .002). Our findings suggest that differences in muscle biochemistry account for part of the interindividual variability in muscle oxygen uptake and whole-body energy metabolism, ie, metabolic rate and substrate oxidation.